Pseudomonas aeruginosa PAO1, PA0317

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0317
Name
Replicon chromosome
Genomic location 356681 - 358075 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentPA0317 is a D-2-hydroxyglutarate dehydrogenase that converts D-2-hydroxyglutarate to 2-ketoglutarate and its alternative substrate D-malate to oxaloacetate
CommentZinc and FAD-dependent enzyme whose electron acceptor is unknown and not oxygen. It uses 1 mole of zinc per mole of protein to orient and activate the substrate upon binding
CommentThe FAD serves as the reaction center for hydride transfer
CommentPaD2HGDH is active with cobalt, manganese, nickel, or cadmium as an alternative metal

Cross-References

RefSeq NP_249008.1
GI 15595514
Affymetrix PA0317_at
Entrez 878323
GenBank AAG03706.1
INSDC AAG03706.1
NCBI Locus Tag PA0317
protein_id(GenBank) gb|AAG03706.1|AE004469_11|gnl|PseudoCAP|PA0317
TIGR NTL03PA00318
UniParc UPI00000C5000
UniProtKB Acc Q9I6H4
UniProtKB ID Q9I6H4_PSEAE
UniRef100 UniRef100_Q9I6H4
UniRef50 UniRef50_F5SNV8
UniRef90 UniRef90_Q9I6H4

Product

Feature Type CDS
Coding Frame 1
Product Name
D-2-hydroxyglutarate dehydrogenase
Product Name Confidence: Class 1
Synonyms D2HGDH
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -13.18
Kyte-Doolittle Hydrophobicity Value -0.079
Molecular Weight (kDa) 51.3
Isoelectric Point (pI) 4.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 549 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000314 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0317

Human Homologs

Ensembl 110, assembly GRCh38.p14
D-2-hydroxyglutarate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:28358]
E-value: 5.3e-72
Percent Identity: 34.4

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Coupling between d-3-phosphoglycerate dehydrogenase and d-2-hydroxyglutarate dehydrogenase drives bacterial l-serine synthesis.
Zhang W, Zhang M, Gao C, Zhang Y, Ge Y, Guo S, Guo X, Zhou Z, Liu Q, Zhang Y, Ma C, Tao F, Xu P
Proc Natl Acad Sci U S A 2017 Sep 5;114(36):E7574-E7582
PubMed ID: 28827360
Kinetic and Bioinformatic Characterization of d-2-Hydroxyglutarate Dehydrogenase from Pseudomonas aeruginosa PAO1.
Quaye JA, Gadda G
Biochemistry 2020 Dec 29;59(51):4833-4844
PubMed ID: 33301690
Uncovering Zn(2+) as a cofactor of FAD-dependent Pseudomonas aeruginosa PAO1 d-2-hydroxyglutarate dehydrogenase.
Quaye JA, Gadda G
J Biol Chem 2023 Mar;299(3):103007
PubMed ID: 36775126
The Pseudomonas aeruginosa PAO1 metallo flavoprotein d-2-hydroxyglutarate dehydrogenase requires Zn(2+) for substrate orientation and activation.
Quaye JA, Gadda G
J Biol Chem 2023 Mar;299(3):103008
PubMed ID: 36775127