Pseudomonas aeruginosa PAO1, PA0845 (cerN)

Gene Ontology

Ontology	Accession	Term	GO Evidence	Evidence Ontology (ECO) Code	Reference	Comments
Molecular Function	GO:0017040	N-acylsphingosine amidohydrolase activity	IMP Inferred from Mutant Phenotype	ECO:0000015 mutant phenotype evidence	10593963	Reviewed by curator
Biological Process	GO:0051872	sphingosine catabolic process	IDA Inferred from Direct Assay	ECO:0000096 electrophoretic mobility shift assay evidence
Biological Process	GO:0051872	sphingosine catabolic process	IDA Inferred from Direct Assay	ECO:0000096 electrophoretic mobility shift assay evidence	24465209	Reviewed by curator
Molecular Function	GO:0017040	N-acylsphingosine amidohydrolase activity	ISM Inferred from Sequence Model Term mapped from: InterPro:PTHR12670	ECO:0000259 match to InterPro signature evidence used in automatic assertion
Biological Process	GO:0046514	ceramide catabolic process	ISM Inferred from Sequence Model Term mapped from: InterPro:PTHR12670	ECO:0000259 match to InterPro signature evidence used in automatic assertion

Functional Classifications Manually Assigned by PseudoCAP

Hypothetical, unclassified, unknown Other PAO1 genes in this class

Pathways

Database	Xref	Pathway	Version	Evidence	PMID
KEGG	pae00600	Sphingolipid metabolism	81.0+/01-23, Jan 17	ECO:0000249 sequence similarity evidence used in automatic assertion
MetaCyc		ceramide degradation	InterPro 5.36-75.0	ECO:0000259 match to InterPro signature evidence used in automatic assertion
MetaCyc		sphingolipid recycling and degradation (yeast)	InterPro 5.36-75.0	ECO:0000259 match to InterPro signature evidence used in automatic assertion
KEGG	pae01100	Metabolic pathways	81.0+/01-23, Jan 17	ECO:0000249 sequence similarity evidence used in automatic assertion

Functional Predictions from Interpro

Analysis	Accession	Description	Interpro Accession	Interpro Description	Amino Acid Start	Amino Acid Stop	E-value
Gene3D	G3DSA:2.60.40.2300	-	IPR038445	Neutral ceramidase, C-terminal domain superfamily	536	670	5.4E-48
Pfam	PF04734	Neutral/alkaline non-lysosomal ceramidase, N-terminal	IPR031329	Neutral/alkaline non-lysosomal ceramidase, N-terminal	29	501	0.0
Pfam	PF17048	Neutral/alkaline non-lysosomal ceramidase, C-terminal	IPR031331	Neutral/alkaline non-lysosomal ceramidase, C-terminal	506	665	2.1E-47
PANTHER	PTHR12670	CERAMIDASE	IPR006823	Neutral/alkaline nonlysosomal ceramidase	282	665	0.0

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.