Pseudomonas aeruginosa PAO1, PA2012 (liuD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2012
Name
liuD
Synonym: mccA
Synonym: gnyA
Synonym: accA
Replicon chromosome
Genomic location 2200685 - 2202652 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250702.1
GI 15597208
Affymetrix PA2012_at
Entrez 879012
GenBank AAG05400.1
INSDC AAG05400.1
NCBI Locus Tag PA2012
protein_id(GenBank) gb|AAG05400.1|AE004627_8|gnl|PseudoCAP|PA2012
TIGR NTL03PA02012
UniParc UPI00000C5558
UniProtKB Acc Q9I299
UniProtKB ID Q9I299_PSEAE
UniRef100 UniRef100_Q9I299
UniRef50 UniRef50_Q9I299
UniRef90 UniRef90_Q9I299

Product

Feature Type CDS
Coding Frame 1
Product Name
methylcrotonyl-CoA carboxylase, alpha-subunit (biotin-containing)
Product Name Confidence: Class 1
Synonyms MccA
alpha subunit of geranoyl-CoA carboxylase, GnyA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.10
Kyte-Doolittle Hydrophobicity Value -0.234
Molecular Weight (kDa) 71.3
Isoelectric Point (pI) 5.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3U9T LIGASE 10/19/11 Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC) 750 kD holoenzyme, free enzyme Pseudomonas aeruginosa 2.9 X-RAY DIFFRACTION 100.0
3U9S LIGASE 10/19/11 Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC) 750 kD holoenzyme, CoA complex Pseudomonas aeruginosa 3.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 643 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003427 (523 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2012
Search term: liuD

Human Homologs

Ensembl 110, assembly GRCh38.p14
methylcrotonyl-CoA carboxylase subunit 1 [Source:HGNC Symbol;Acc:HGNC:6936]
E-value: 4.1e-163
Percent Identity: 45.9

References

The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa.
Díaz-Pérez AL, Zavala-Hernández AN, Cervantes C, Campos-García J
Appl Environ Microbiol 2004 Sep;70(9):5102-10
PubMed ID: 15345388
Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa.
Förster-Fromme K, Höschle B, Mack C, Bott M, Armbruster W, Jendrossek D
Appl Environ Microbiol 2006 Jul;72(7):4819-28
PubMed ID: 16820476
Lipid synthesis in mycobacteria: characterization of the biotin carboxyl carrier protein genes from Mycobacterium leprae and M. tuberculosis.
Norman E, De Smet KA, Stoker NG, Ratledge C, Wheeler PR, Dale JW
J Bacteriol 1994 May;176(9):2525-31
PubMed ID: 7909542