Pseudomonas aeruginosa PAO1, PA2407 (fpvC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2407
Name
fpvC
Replicon chromosome
Genomic location 2690160 - 2691113 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 1XVL (Mn transporter from Synechocystis 6803, root-mean-square deviation: 0.9 angstroms). Sequence similarity to 1PSZ (97%/23,84%) (ZN). Final predicted ligand: metal Ion (PMID: 31631799)
CommentPart ABC transporter complex FpvWXYZCDEF coded by operons fpvWXYZCDEF and fpvF.

Cross-References

RefSeq NP_251097.1
GI 15597603
Affymetrix PA2407_at
DNASU PaCD00007047
Entrez 879560
GenBank AAG05795.1
INSDC AAG05795.1
NCBI Locus Tag PA2407
protein_id(GenBank) gb|AAG05795.1|AE004668_5|gnl|PseudoCAP|PA2407
TIGR NTL03PA02407
UniParc UPI00000C56B6
UniProtKB Acc Q9I174
UniProtKB ID Q9I174_PSEAE
UniRef100 UniRef100_A0A069PX18
UniRef50 UniRef50_Q884F2
UniRef90 UniRef90_A6V577

Product

Feature Type CDS
Coding Frame 1
Product Name
FpvC
Product Name Confidence: Class 1
Synonyms probable adhesion protein
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 6.68
Kyte-Doolittle Hydrophobicity Value -0.032
Molecular Weight (kDa) 34.3
Isoelectric Point (pI) 9.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6R6K PROTEIN TRANSPORT 03/27/19 Structure of a FpvC mutant from pseudomonas aeruginosa Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 97.9
6R5S TRANSPORT PROTEIN 03/25/19 Structure of the SBP FpvC from pseudomonas aeruginosa in complex with Fe(II) Pseudomonas aeruginosa 2.75 X-RAY DIFFRACTION 100.0
6R3Z TRANSPORT PROTEIN 03/21/19 Structure of the SBP FpvC in complex with Ni2+ ion from P. aeruginosa in P212121 space group Pseudomonas aeruginosa PAO1 1.65 X-RAY DIFFRACTION 100.0
6R44 TRANSPORT PROTEIN 03/21/19 Structure of the SBP FpvC in complex with Ni2+ ion from P.aeruginosa from P21 space group Pseudomonas aeruginosa PAO1 1.99 X-RAY DIFFRACTION 100.0
6RU4 TRANSPORT PROTEIN 05/27/19 Structure of the SBP FpvC from pseudomonas aeruginosa in complex with Mn2+ Pseudomonas aeruginosa PAO1 2.49 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 473 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003125 (477 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2407
Search term: fpvC
Search term: FpvC

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH.
Ganne G, Brillet K, Basta B, Roche B, Hoegy F, Gasser V, Schalk IJ
ACS Chem Biol 2017 Apr 21;12(4):1056-1065
PubMed ID: 28192658
An ABC transporter with two periplasmic binding proteins involved in iron acquisition in Pseudomonas aeruginosa.
Brillet K, Ruffenach F, Adams H, Journet L, Gasser V, Hoegy F, Guillon L, Hannauer M, Page A, Schalk IJ
ACS Chem Biol 2012 Dec 21;7(12):2036-45
PubMed ID: 23009327
The Two-Operon-Coded ABC Transporter Complex FpvWXYZCDEF is Required for Pseudomonas aeruginosa Growth and Virulence Under Iron-Limiting Conditions.
Gao L, Guo Z, Wang Y, Wang Y, Wang K, Li B, Shen L
J Membr Biol 2018 Feb;251(1):91-104
PubMed ID: 28975384
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799