Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3186
|
Name |
oprB
|
Replicon | chromosome |
Genomic location | 3575912 - 3577276 (- strand) |
Transposon Mutants | 5 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 4 transposon mutants in orthologs |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_251876.1 |
GI | 15598382 |
Affymetrix | PA3186_oprB_s_at |
Entrez | 882788 |
GenBank | AAG06574.1 |
INSDC | AAG06574.1 |
NCBI Locus Tag | PA3186 |
protein_id(GenBank) | gb|AAG06574.1|AE004742_10|gnl|PseudoCAP|PA3186 |
TIGR | NTL03PA03186 |
UniParc | UPI0000131F64 |
UniProtKB Acc | Q51485 |
UniProtKB ID | PORB_PSEAE |
UniRef100 | UniRef100_Q51485 |
UniRef50 | UniRef50_Q51485 |
UniRef90 | UniRef90_Q51485 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Glucose/carbohydrate outer membrane porin OprB precursor
|
Synonyms |
D1 carbohydrate-selective porin OprB |
Evidence for Translation |
Detected using LC-MS/MS (50.75 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -7.16 |
Kyte-Doolittle Hydrophobicity Value | -0.537 |
Molecular Weight (kDa) | 50.8 |
Isoelectric Point (pI) | 5.48 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 51 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002430 (700 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3186
Search term: oprB
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Cloning and nucleotide sequence of the Pseudomonas aeruginosa glucose-selective OprB porin gene and distribution of OprB within the family Pseudomonadaceae.
Wylie JL, Worobec EA
Eur J Biochem 1994 Mar 1;220(2):505-12
PubMed ID: 8125108
|
The OprB porin plays a central role in carbohydrate uptake in Pseudomonas aeruginosa.
Wylie JL, Worobec EA
J Bacteriol 1995 Jun;177(11):3021-6
PubMed ID: 7768797
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|