Pseudomonas aeruginosa PAO1, PA3204 (cpxR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3204
Name
cpxR
Replicon chromosome
Genomic location 3594581 - 3595258 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251894.1
GI 15598400
Affymetrix PA3204_at
Entrez 882544
GenBank AAG06592.1
INSDC AAG06592.1
NCBI Locus Tag PA3204
protein_id(GenBank) gb|AAG06592.1|AE004744_7|gnl|PseudoCAP|PA3204
TIGR NTL03PA03204
UniParc UPI00000C594A
UniProtKB Acc Q9HZ36
UniProtKB ID Q9HZ36_PSEAE
UniRef100 UniRef100_W0YUZ4
UniRef50 UniRef50_Q4KGB4
UniRef90 UniRef90_M4WZL9

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component response regulator CpxR
Product Name Confidence: Class 1
Synonyms probable two-component response regulator
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.62
Kyte-Doolittle Hydrophobicity Value -0.280
Molecular Weight (kDa) 24.8
Isoelectric Point (pI) 5.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
Pseudomonas aeruginosa CpxR CpxR is directly involved in activation of expression of RND efflux pump MexAB-OprM in P. aeruginosa. CpxR is required to enhance mexAB-oprM expression and drug resistance, in the absence of repressor MexR. [PMID:27736975] ARO:3004054 protein homolog model Perfect 444.1 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 622 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002411 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3204
Search term: cpxR

Human Homologs

References

The cpx proteins of Escherichia coli K12. Structure of the cpxA polypeptide as an inner membrane component.
Weber RF, Silverman PM
J Mol Biol 1988 Sep 20;203(2):467-78
PubMed ID: 3058985
CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa.
Tian ZX, Yi XX, Cho A, O'Gara F, Wang YP
PLoS Pathog 2016 Oct;12(10):e1005932
PubMed ID: 27736975