Pseudomonas aeruginosa PAO1, PA3385 (amrZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3385
Name
amrZ
Synonym: algZ
Replicon chromosome
Genomic location 3791347 - 3791673 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252075.1
GI 15598581
Affymetrix PA3385_at
DNASU PaCD00007486
Entrez 878714
GenBank AAG06773.1
INSDC AAG06773.1
NCBI Locus Tag PA3385
protein_id(GenBank) gb|AAG06773.1|AE004760_1|gnl|PseudoCAP|PA3385
TIGR NTL03PA03385
UniParc UPI00000D4234
UniProtKB Acc G3XCY4
UniProtKB ID G3XCY4_PSEAE
UniRef100 UniRef100_W0YXA2
UniRef50 UniRef50_F8H3I4
UniRef90 UniRef90_W0YXA2

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate and motility regulator Z
Product Name Confidence: Class 1
Synonyms AlgZ
Evidence for Translation
Charge (pH 7) 1.19
Kyte-Doolittle Hydrophobicity Value -0.534
Molecular Weight (kDa) 12.3
Isoelectric Point (pI) 7.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3QOQ Transcription/DNA 02/10/11 Crystal Structure of the Transcription Factor AmrZ in Complex with the 18 Base Pair amrZ1 Binding Site Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.1 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002243 (527 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3385
Search term: amrZ

Human Homologs

References

The Pseudomonas aeruginosa ribbon-helix-helix DNA-binding protein AlgZ (AmrZ) controls twitching motility and biogenesis of type IV pili.
Baynham PJ, Ramsey DM, Gvozdyev BV, Cordonnier EM, Wozniak DJ
J Bacteriol 2006 Jan;188(1):132-40
PubMed ID: 16352829
Binding of Pseudomonas aeruginosa AlgZ to sites upstream of the algZ promoter leads to repression of transcription.
Ramsey DM, Baynham PJ, Wozniak DJ
J Bacteriol 2005 Jul;187(13):4430-43
PubMed ID: 15968052
Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT.
Wozniak DJ, Sprinkle AB, Baynham PJ
J Bacteriol 2003 Dec;185(24):7297-300
PubMed ID: 14645293
Pseudomonas aeruginosa AlgZ, a ribbon-helix-helix DNA-binding protein, is essential for alginate synthesis and algD transcriptional activation.
Baynham PJ, Brown AL, Hall LL, Wozniak DJ
Mol Microbiol 1999 Sep;33(5):1069-80
PubMed ID: 10476040
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A
Proc Natl Acad Sci U S A 2017 Jul 18;114(29):7707-7712
PubMed ID: 28673999