Pseudomonas aeruginosa PAO1, PA3649 (mucP)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3649
Name	mucP Synonym: rseP
Replicon	chromosome
Genomic location	4087526 - 4088878 (- strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_252339.1
GI	15598845
Affymetrix	PA3649_at
Entrez	880507
GenBank	AAG07037.1
INSDC	CEI78504.1
NCBI Locus Tag	PA3649
protein_id(GenBank)	gb|AAG07037.1|AE004785_1|gnl|PseudoCAP|PA3649
TIGR	NTL03PA03649
UniParc	UPI0000139266
UniProtKB Acc	A5JIY6
UniProtKB Acc	A5JIY6
UniProtKB ID	Y3649_PSEAE
UniProtKB ID	A5JIY6_PSEAI
UniRef100	UniRef100_Q9HXY3
UniRef50	UniRef50_Q9HXY3
UniRef90	UniRef90_Q9HXY3

Product

Feature Type	CDS
Coding Frame	1
Product Name	MucP Product Name Confidence: Class 4
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-0.33
Kyte-Doolittle Hydrophobicity Value	0.268
Molecular Weight (kDa)	48.5
Isoelectric Point (pI)	6.90

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7XFU	LYASE	04/02/22	MucP PDZ2 domain with peptide GPAVLA	Pseudomonas aeruginosa PAO1; synthetic construct	1.53	X-RAY DIFFRACTION	98.9
7XFS	LYASE	04/02/22	MucP PDZ1 domain	Pseudomonas aeruginosa PAO1	1.06	X-RAY DIFFRACTION	100.0
7XFT	LYASE	04/02/22	MucP PDZ2 domain	Pseudomonas aeruginosa PAO1	1.21	X-RAY DIFFRACTION	98.9

Virulence Evidence

Source 1
Database	VFDB
Category	Alginate regulation
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002025 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3649 Search term: mucP Search term: MucP

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity. Damron FH, Yu HD J Bacteriol 2011 Jan;193(1):286-91 PubMed ID: 21036998

Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa. Qiu D, Eisinger VM, Rowen DW, Yu HD Proc Natl Acad Sci U S A 2007 May 8;104(19):8107-12 PubMed ID: 17470813

Cell-surface signaling in Pseudomonas: stress responses, iron transport, and pathogenicity. Llamas MA, Imperi F, Visca P, Lamont IL FEMS Microbiol Rev 2014 Jul;38(4):569-97 PubMed ID: 24923658

Differential proteolysis of sigma regulators controls cell-surface signalling in Pseudomonas aeruginosa. Draper RC, Martin LW, Beare PA, Lamont IL Mol Microbiol 2011 Dec;82(6):1444-53 PubMed ID: 22040024