Pseudomonas aeruginosa UCBPP-PA14, PA14_43090 (tap)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_43090
Name
tap
Replicon chromosome
Genomic location 3834632 - 3835372 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
CommentHcp Secretion Island-II-encoded type VI secretion system (H2-T6SS)

Cross-References

RefSeq YP_791593.1
GI 116049602
Entrez 4381810
INSDC ABJ10838.1
NCBI Locus Tag PA14_43090
UniParc UPI000045D1AA
UniProtKB Acc Q02KE3
UniProtKB ID Q02KE3_PSEAB
UniRef100 UniRef100_Q02KE3
UniRef50 UniRef50_Q87U71
UniRef90 UniRef90_Q02KE3

Product

Feature Type CDS
Coding Frame 1
Product Name
Tap
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -2.87
Kyte-Doolittle Hydrophobicity Value -0.259
Molecular Weight (kDa) 27.9
Isoelectric Point (pI) 6.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse acute lung Infection model
Infection model Mouse burn Infection model
Reference 19497948
Source 2
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration model
Reference 19497948

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG014914 (121 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_43090
Search term: tap
Search term: Tap

Human Homologs

References

Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis.
Lesic B, Starkey M, He J, Hazan R, Rahme LG
Microbiology (Reading) 2009 Sep;155(Pt 9):2845-2855
PubMed ID: 19497948
The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells.
Sana TG, Hachani A, Bucior I, Soscia C, Garvis S, Termine E, Engel J, Filloux A, Bleves S
J Biol Chem 2012 Aug 3;287(32):27095-105
PubMed ID: 22665491
An rhs gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14.
Jones C, Hachani A, Manoli E, Filloux A
J Bacteriol 2014 Feb;196(4):800-10
PubMed ID: 24317402
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A
Proc Natl Acad Sci U S A 2017 Jul 18;114(29):7707-7712
PubMed ID: 28673999