Pseudomonas aeruginosa PAO1, PA0085 (hcp1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0085
Name
hcp1
Replicon chromosome
Genomic location 103350 - 103838 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248775.1
GI 15595283
Affymetrix PA0085_at
DNASU PaCD00006037
Entrez 879446
GenBank AAG03475.1
INSDC AAG03475.1
NCBI Locus Tag PA0085
protein_id(GenBank) gb|AAG03475.1|AE004447_8|gnl|PseudoCAP|PA0085
TIGR NTL03PA00086
UniParc UPI00000C4F26
UniProtKB Acc Q9I747
UniProtKB ID HCP1_PSEAE
UniRef100 UniRef100_Q9I747
UniRef50 UniRef50_Q9I747
UniRef90 UniRef90_Q9I747

Product

Feature Type CDS
Coding Frame 1
Product Name
Hcp1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis, mass spectral analysis and protein mass mapping (PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.32
Kyte-Doolittle Hydrophobicity Value -0.415
Molecular Weight (kDa) 17.4
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4KSS PROTEIN TRANSPORT 05/17/13 Crystal Structure of Vibrio cholerae ATPase GspsE Hexamer VIBRIO CHOLERAE O1, PSEUDOMONAS AERUGINOSA 7.58 X-RAY DIFFRACTION 100.0
4KSR PROTEIN TRANSPORT 05/17/13 Crystal Structure of the Vibrio cholerae ATPase GspE Hexamer VIBRIO CHOLERAE O1, PSEUDOMONAS AERUGINOSA 4.2 X-RAY DIFFRACTION 100.0
1Y12 structural genomics, unknown function 11/16/04 Structure of a hemolysin-coregulated protein from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 1.95 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 75 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000084 (777 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0085
Search term: hcp1
Search term: Hcp1

References

A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 2006 Jun;312(5779):1526-30
PubMed ID: 16763151
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142