Pseudomonas aeruginosa PAO1, PA0259 (tla3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0259
Name
tla3
Synonym: tli3
Replicon chromosome
Genomic location 289562 - 291004 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentIn a previous release, we updated the start site to 291154 based on a recombinant tla3 gene with this start site being used in E. coli. There is no evidence for 291154 being the translation initiation site in P. aeruginosa so it have been reverted back to 291004.
CommentTla3 is localized in the cytoplasm of P. aeruginosa and is required for Tle3 secretion and antibacterial activity in P. aeruginosa. Direct interaction with Tle3 and VgrG2b

Cross-References

RefSeq NP_248950.1
GI 15595456
Affymetrix PA0259_at
Entrez 881892
GenBank AAG03648.1
INSDC AAG03648.1
NCBI Locus Tag PA0259
protein_id(GenBank) gb|AAG03648.1|AE004464_1|gnl|PseudoCAP|PA0259
TIGR NTL03PA00260
UniParc UPI00000C4FC8
UniProtKB Acc Q9I6N0
UniProtKB ID Q9I6N0_PSEAE
UniRef100 UniRef100_Q9I6N0
UniRef50 UniRef50_Q9I6N0
UniRef90 UniRef90_Q9I6N0

Product

Feature Type CDS
Coding Frame 1
Product Name
Type 6 lipase adaptor, Tla3
Product Name Confidence: Class 1
Synonyms Tli3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.32
Kyte-Doolittle Hydrophobicity Value -0.317
Molecular Weight (kDa) 52.4
Isoelectric Point (pI) 5.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 7 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000257 (262 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0259
Search term: tla3

Human Homologs

References

Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
Nature 2013 Apr 25;496(7446):508-12
PubMed ID: 23552891
A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in Pseudomonas aeruginosa.
Berni B, Soscia C, Djermoun S, Ize B, Bleves S
Front Microbiol 2019;10:1218
PubMed ID: 31231326