Pseudomonas aeruginosa PAO1, PA0261 (tli3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0261
Name
tli3
Replicon chromosome
Genomic location 293301 - 293798 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentThe annoted ATG has been checked in P. aeruginosa and is correct. Directly interacts with Tle3. Cognate immunity protein for Tle3 (in P. aeruginosa and in the heterologous host E. coli) and for VgrG2b C-Terminal domain (in the heterologous host E. coli) (PMID:31231326).

Cross-References

RefSeq NP_248952.1
GI 15595458
Affymetrix PA0261_at
DNASU PaCD00006118
Entrez 881884
GenBank AAG03650.1
INSDC AAG03650.1
NCBI Locus Tag PA0261
protein_id(GenBank) gb|AAG03650.1|AE004464_3|gnl|PseudoCAP|PA0261
TIGR NTL03PA00262
UniParc UPI00000C4FCA
UniProtKB Acc Q9I6M8
UniProtKB ID Q9I6M8_PSEAE
UniRef100 UniRef100_W0YJQ4
UniRef50 UniRef50_Q02UH2
UniRef90 UniRef90_A6V0P9

Product

Feature Type CDS
Coding Frame 1
Product Name
Type 6 lipase immunity, Tli3
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.77
Kyte-Doolittle Hydrophobicity Value -0.276
Molecular Weight (kDa) 18.4
Isoelectric Point (pI) 8.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000259 (251 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0261
Search term: tli3

Human Homologs

References

A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in Pseudomonas aeruginosa.
Berni B, Soscia C, Djermoun S, Ize B, Bleves S
Front Microbiol 2019;10:1218
PubMed ID: 31231326