Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0395
|
Name |
pilT
|
Replicon | chromosome |
Genomic location | 436570 - 437604 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_249086.1 |
GI | 15595592 |
Affymetrix | PA0395_pilT_at |
DNASU | PaCD00006170 |
Entrez | 878389 |
GenBank | AAG03784.1 |
INSDC | AAG03784.1 |
NCBI Locus Tag | PA0395 |
protein_id(GenBank) | gb|AAG03784.1|AE004477_1|gnl|PseudoCAP|PA0395 |
TIGR | NTL03PA00396 |
UniParc | UPI0000131AD1 |
UniProtKB Acc | P24559 |
UniProtKB ID | PILT_PSEAE |
UniRef100 | UniRef100_P24559 |
UniRef50 | UniRef50_P24559 |
UniRef90 | UniRef90_P24559 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
twitching motility protein PilT
|
Synonyms |
type 4 fimbrial biogenesis protein PilT |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -0.64 |
Kyte-Doolittle Hydrophobicity Value | -0.114 |
Molecular Weight (kDa) | 38.0 |
Isoelectric Point (pI) | 6.87 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3JVV | ATP binding protein | 09/17/09 | Crystal Structure of P. aeruginosa PilT with bound AMP-PCP | Pseudomonas aeruginosa | 2.6 | X-RAY DIFFRACTION | 100.0 |
3JVU | ATP binding protein | 09/17/09 | Crystal structure of unliganded P. aeruginosa PilT | Pseudomonas aeruginosa | 3.1 | X-RAY DIFFRACTION | 100.0 |
Source 1 | |
Database | VFDB : VF0082 |
Category | Type IV pili biosynthesis |
Evidence |
|
Host Organism | |
Notes | |
Reference | 9224879 |
Source 2 | |
Database | PseudoCAP |
Category | Type IV pili biosynthesis |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Galleria mellonella |
Notes | Mortality greater after inoculation with wild-type PAO1 compared with pilT mutant |
Infection model | Galleria mellonella killing assay |
Reference | 16849183 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 517 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000386 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0395
Search term: pilT
Search term: twitching motility protein PilT
|
Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria.
Whitchurch CB, Hobbs M, Livingston SP, Krishnapillai V, Mattick JS
Gene 1991 May 15;101(1):33-44
PubMed ID: 1676385
|
PilS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa.
Hobbs M, Collie ES, Free PD, Livingston SP, Mattick JS
Mol Microbiol 1993 Mar;7(5):669-82
PubMed ID: 8097014
|
Characterization of a gene, pilU, required for twitching motility but not phage sensitivity in Pseudomonas aeruginosa.
Whitchurch CB, Mattick JS
Mol Microbiol 1994 Sep;13(6):1079-91
PubMed ID: 7854122
|
Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia.
Comolli JC, Hauser AR, Waite L, Whitchurch CB, Mattick JS, Engel JN
Infect Immun 1999 Jul;67(7):3625-30
PubMed ID: 10377148
|