Pseudomonas aeruginosa PAO1, PA0425 (mexA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0425
Name
mexA
Replicon chromosome
Genomic location 472024 - 473175 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249116.1
GI 15595622
Affymetrix PA0425_mexA_at
Entrez 877855
GenBank AAG03814.1
INSDC AAD45627.1
NCBI Locus Tag PA0425
protein_id(GenBank) gb|AAG03814.1|AE004479_9|gnl|PseudoCAP|PA0425
TIGR NTL03PA00426
UniParc UPI000012F021
UniProtKB Acc P52477
UniProtKB ID MEXA_PSEAE
UniRef100 UniRef100_P52477
UniRef50 UniRef50_P52477
UniRef90 UniRef90_P52477

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux membrane fusion protein MexA precursor
Product Name Confidence: Class 1
Evidence for Translation
Detected using 2-DE, MALDI-TOF/MS analysis (40.97 kDa, pI 8.8, PMID:16335974)
Detected using LC-MS/MS (40.97 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.22
Kyte-Doolittle Hydrophobicity Value -0.229
Molecular Weight (kDa) 41.0
Isoelectric Point (pI) 9.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Outer Membrane Class 1
Additional evidence for subcellular localization
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
MexA MexA is the membrane fusion protein of the MexAB-OprM multidrug efflux complex. ARO:3000377 protein homolog model Perfect 0.0 1.0 1.1.8

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1VF7 MEMBRANE PROTEIN 04/09/04 Crystal structure of the membrane fusion protein, MexA of the multidrug transporter PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0
1T5E TRANSPORT PROTEIN 05/04/04 The structure of MexA PSEUDOMONAS AERUGINOSA 3.0 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 394 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000416 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0425
Search term: mexA

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 30275
Genomic Position 472501
Position in ORF 478(1152)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 31900
Genomic Position 472945
Position in ORF 922(1152)
Reference 14617778

Transposon Insertions in Orthologs

References

Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine.
Poole K, Heinrichs DE, Neshat S
Mol. Microbiol. 1993 Nov;10(3):529-44
PubMed ID: 7968531
Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.
Li XZ, Nikaido H, Poole K
Antimicrob. Agents Chemother. 1995 Sep;39(9):1948-53
PubMed ID: 8540696
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J. Proteome Res. ;4(6):2257-65
PubMed ID: 16335974
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344