Pseudomonas aeruginosa PAO1, PA0426 (mexB)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 473191 - 476331 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 8 transposon mutants in orthologs


RefSeq NP_249117.1
GI 15595623
Affymetrix PA0426_mexB_at
Entrez 877852
GenBank AAG03815.1
INSDC AAD45628.1
NCBI Locus Tag PA0426
protein_id(GenBank) gb|AAG03815.1|AE004479_10|gnl|PseudoCAP|PA0426
UniParc UPI000012F022
UniProtKB Acc P52002
UniRef100 UniRef100_P52002
UniRef50 UniRef50_P52002
UniRef90 UniRef90_P52002


Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexB
Product Name Confidence: Class 1
Synonyms RND multidrug efflux transporter MexB
Evidence for Translation
Detected using LC-MS/MS (112.79 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.89
Kyte-Doolittle Hydrophobicity Value 0.274
Molecular Weight (kDa) 112.8
Isoelectric Point (pI) 5.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
adeG AdeG is the inner membrane transporter of the AdeFGH multidrug efflux complex. ARO:3000778 protein homolog model Strict 0.0 0.999044 1.1.8

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 488 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000417 (2173 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0426
Search term: mexB


Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine.
Poole K, Heinrichs DE, Neshat S
Mol. Microbiol. 1993 Nov;10(3):529-44
PubMed ID: 7968531
Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.
Li XZ, Nikaido H, Poole K
Antimicrob. Agents Chemother. 1995 Sep;39(9):1948-53
PubMed ID: 8540696
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344