Pseudomonas aeruginosa PAO1, PA0427 (oprM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0427
Name
oprM
Replicon chromosome
Genomic location 476333 - 477790 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249118.1
GI 15595624
Affymetrix PA0427_oprM_at
Entrez 877851
GenBank AAG03816.1
INSDC AAA74438.1
NCBI Locus Tag PA0427
protein_id(GenBank) gb|AAG03816.1|AE004479_11|gnl|PseudoCAP|PA0427
TIGR NTL03PA00428
UniParc UPI0000130D9F
UniProtKB Acc Q51487
UniProtKB ID OPRM_PSEAE
UniRef100 UniRef100_Q51487
UniRef50 UniRef50_Q51487
UniRef90 UniRef90_Q51487

Product

Feature Type CDS
Coding Frame 1
Product Name
Major intrinsic multiple antibiotic resistance efflux outer membrane protein OprM precursor
Product Name Confidence: Class 1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (52.60 kDa, PMID:21751344)
Charge (pH 7) -2.79
Kyte-Doolittle Hydrophobicity Value -0.215
Molecular Weight (kDa) 52.6
Isoelectric Point (pI) 5.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
OprM OprM is an outer membrane factor protein found in Pseudomonas aeruginosa and Burkholderia vietnamiensis. It is part of the MexAB-OprM, MexVW-OprM, MexXY-OprM and the AmrAB-OprM complex. ARO:3000379 protein homolog model Perfect 0.0 1.0 1.1.8

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3D5K MEMBRANE PROTEIN 05/16/08 Crystal structure of the OprM channel in a non-symmetrical space group PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0
4Y1K MEMBRANE PROTEIN 02/07/15 PALMITOYLATED OPRM OUTER MEMBRANE FACTOR PSEUDOMONAS AERUGINOSA 3.8 X-RAY DIFFRACTION 100.0
1WP1 MEMBRANE PROTEIN 08/28/04 Crystal structure of the drug-discharge outer membrane protein, OprM PSEUDOMONAS AERUGINOSA 2.56 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 316 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000418 (559 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0427
Search term: oprM

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 6089
Genomic Position 477717
Position in ORF 1385(1458)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 7642
Genomic Position 477135
Position in ORF 803(1458)
Reference 14617778

Transposon Insertions in Orthologs

References

Evidence for the location of OprM in the Pseudomonas aeruginosa outer membrane.
Gotoh N, Itoh N, Yamada H, Nishino T
FEMS Microbiol. Lett. 1994 Oct;122(3):309-12
PubMed ID: 7988873
Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon.
Poole K, Krebes K, McNally C, Neshat S
J. Bacteriol. 1993 Nov;175(22):7363-72
PubMed ID: 8226684
Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.
Li XZ, Nikaido H, Poole K
Antimicrob. Agents Chemother. 1995 Sep;39(9):1948-53
PubMed ID: 8540696
The outer membrane protein OprM of Pseudomonas aeruginosa is encoded by oprK of the mexA-mexB-oprK multidrug resistance operon.
Gotoh N, Tsujimoto H, Poole K, Yamagishi J, Nishino T
Antimicrob. Agents Chemother. 1995 Nov;39(11):2567-9
PubMed ID: 8585747
The role of mex-gene products in antibiotic extrusion in Pseudomonas aeruginosa.
Yoneyama H, Ocaktan A, Tsuda M, Nakae T
Biochem. Biophys. Res. Commun. 1997 Apr;233(3):611-8
PubMed ID: 9168899
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344