Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0516
|
Name |
nirF
|
Replicon | chromosome |
Genomic location | 576498 - 577676 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_249207.1 |
GI | 15595713 |
Affymetrix | PA0516_nirF_at |
Entrez | 882198 |
GenBank | AAG03905.1 |
INSDC | AAG03905.1 |
NCBI Locus Tag | PA0516 |
protein_id(GenBank) | gb|AAG03905.1|AE004488_9|gnl|PseudoCAP|PA0516 |
TIGR | NTL03PA00517 |
UniParc | UPI00001301B2 |
UniProtKB Acc | Q51480 |
UniProtKB ID | NIRF_PSEAE |
UniRef100 | UniRef100_Q51480 |
UniRef50 | UniRef50_Q51480 |
UniRef90 | UniRef90_Q51480 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
heme d1 biosynthesis protein NirF
|
Synonyms |
cytochrome oxidase |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (43.34 kDa, PMID:21751344)
|
Charge (pH 7) | -2.62 |
Kyte-Doolittle Hydrophobicity Value | -0.259 |
Molecular Weight (kDa) | 43.3 |
Isoelectric Point (pI) | 6.52 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6TV9 | UNKNOWN FUNCTION | 01/09/20 | Heme d1 biosynthesis associated Protein NirF in complex with dihydro-heme d1 | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.893 | X-RAY DIFFRACTION | 100.0 |
6TV2 | UNKNOWN FUNCTION | 01/08/20 | Heme d1 biosynthesis associated Protein NirF | Pseudomonas aeruginosa PAO1 | 1.561 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 69 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000494 (709 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0516
Search term: nirF
Search term: heme d1 biosynthesis protein NirF
|
Sequencing and characterization of the downstream region of the genes encoding nitrite reductase and cytochrome c-551 (nirSM) from Pseudomonas aeruginosa: identification of the gene necessary for biosynthesis of heme d1.
Kawasaki S, Arai H, Igarashi Y, Kodama T
Gene 1995 Dec 29;167(1-2):87-91
PubMed ID: 8566817
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|