Pseudomonas aeruginosa PAO1, PA0527 (dnr)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0527
Name
dnr
Replicon chromosome
Genomic location 585980 - 586663 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249218.1
GI 15595724
Affymetrix PA0527_dnr_at
DNASU PaCD00006222
Entrez 882180
GenBank AAG03916.1
INSDC AAG03916.1
NCBI Locus Tag PA0527
protein_id(GenBank) gb|AAG03916.1|AE004489_8|gnl|PseudoCAP|PA0527
TIGR NTL03PA00528
UniParc UPI00000D420C
UniProtKB Acc G3XCW6
UniProtKB ID G3XCW6_PSEAE
UniRef100 UniRef100_G3XCW6
UniRef50 UniRef50_G3XCW6
UniRef90 UniRef90_G3XCW6

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator Dnr
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.69
Kyte-Doolittle Hydrophobicity Value -0.170
Molecular Weight (kDa) 25.9
Isoelectric Point (pI) 6.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3DKW TRANSCRIPTION REGULATOR 06/26/08 Crystal Structure of DNR from Pseudomonas aeruginosa. Pseudomonas aeruginosa 3.6 X-RAY DIFFRACTION 100.0
2Z69 TRANSCRIPTION REGULATOR 07/24/07 Crystal Structure of the sensor domain of the transcriptional regulator DNR from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 454 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000511 (369 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0527
Search term: dnr

Human Homologs

References

Expression of the nir and nor genes for denitrification of Pseudomonas aeruginosa requires a novel CRP/FNR-related transcriptional regulator, DNR, in addition to ANR.
Arai H, Igarashi Y, Kodama T
FEBS Lett 1995 Aug 28;371(1):73-6
PubMed ID: 7664887