Pseudomonas aeruginosa PAO1, PA0618

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0618
Name
Replicon chromosome
Genomic location 677406 - 678293 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249309.1
GI 15595815
Affymetrix PA0618_at
DNASU PaCD00006273
Entrez 879222
GenBank AAG04007.1
INSDC AAG04007.1
NCBI Locus Tag PA0618
protein_id(GenBank) gb|AAG04007.1|AE004498_2|gnl|PseudoCAP|PA0618
TIGR NTL03PA00619
UniParc UPI00000D4232
UniProtKB Acc G3XCX5
UniProtKB ID G3XCX5_PSEAE
UniRef100 UniRef100_Q9S578
UniRef50 UniRef50_Q1I685
UniRef90 UniRef90_Q9S578

Product

Feature Type CDS
Coding Frame 1
Product Name
probable bacteriophage protein
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected using LC-MS/MS (31.96 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.57
Kyte-Doolittle Hydrophobicity Value 0.018
Molecular Weight (kDa) 31.9
Isoelectric Point (pI) 4.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6U5B ANTIMICROBIAL PROTEIN 08/27/19 CryoEM Structure of Pyocin R2 - precontracted - baseplate Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.5 ELECTRON MICROSCOPY 100.0
6U5K ANTIMICROBIAL PROTEIN 08/27/19 CryoEM Structure of Pyocin R2 - postcontracted - baseplate Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.5 ELECTRON MICROSCOPY 100.0
5CES STRUCTURAL PROTEIN 07/07/15 C-terminal domain of the R-type pyocin baseplate protein PA0618 Pseudomonas aeruginosa PAO1 2.102 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 92 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000601 (370 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0618

Human Homologs

References

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344