Pseudomonas aeruginosa PAO1, PA0622

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0622
Name
Replicon chromosome
Genomic location 681394 - 682554 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_249313.1
GI 15595819
Affymetrix PA0622_at
DNASU PaCD00006275
Entrez 877994
GenBank AAG04011.1
INSDC AAG04011.1
NCBI Locus Tag PA0622
protein_id(GenBank) gb|AAG04011.1|AE004498_6|gnl|PseudoCAP|PA0622
TIGR NTL03PA00623
UniParc UPI00000D4266
UniProtKB Acc G3XD39
UniProtKB ID G3XD39_PSEAE
UniRef100 UniRef100_G3XD39
UniRef50 UniRef50_Q02TE1
UniRef90 UniRef90_Q02TE1

Product

Feature Type CDS
Coding Frame 1
Product Name
probable bacteriophage protein
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (41.21 kDa, PMID:21751344)
Charge (pH 7) -5.36
Kyte-Doolittle Hydrophobicity Value -0.012
Molecular Weight (kDa) 41.2
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6U5J ANTIMICROBIAL PROTEIN 08/27/19 CryoEM Structure of Pyocin R2 - postcontracted - collar Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.5 ELECTRON MICROSCOPY 100.0
6U5F ANTIMICROBIAL PROTEIN 08/27/19 CryoEM Structure of Pyocin R2 - precontracted - collar Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.8 ELECTRON MICROSCOPY 100.0
6U5K ANTIMICROBIAL PROTEIN 08/27/19 CryoEM Structure of Pyocin R2 - postcontracted - baseplate Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.5 ELECTRON MICROSCOPY 100.0
6U5B ANTIMICROBIAL PROTEIN 08/27/19 CryoEM Structure of Pyocin R2 - precontracted - baseplate Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.5 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 152 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000605 (327 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0622

Human Homologs

References

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878