Pseudomonas aeruginosa PAO1, PA0666 (anmK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0666
Name
anmK
Replicon chromosome
Genomic location 717635 - 718726 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249357.1
GI 15595863
Affymetrix PA0666_at
DNASU PaCD00006304
Entrez 882072
GenBank AAG04055.1
INSDC AAG04055.1
NCBI Locus Tag PA0666
protein_id(GenBank) gb|AAG04055.1|AE004501_7|gnl|PseudoCAP|PA0666
TIGR NTL03PA00667
UniParc UPI00000C50EF
UniProtKB Acc Q9I5Q5
UniProtKB ID ANMK_PSEAE
UniRef100 UniRef100_Q9I5Q5
UniRef50 UniRef50_Q7NV67
UniRef90 UniRef90_Q9I5Q5

Product

Feature Type CDS
Coding Frame 1
Product Name
1,6-Anhydro-N-acetylmuramic acid kinase, AnmK
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.25
Kyte-Doolittle Hydrophobicity Value -0.026
Molecular Weight (kDa) 39.1
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4MO4 TRANSFERASE 09/11/13 Crystal structure of AnmK bound to AMPPCP Pseudomonas aeruginosa 1.67 X-RAY DIFFRACTION 100.0
3QBW TRANSFERASE 01/14/11 Crystal structure of pseudomonas aeruginosa 1,6-anhydro-n-actetylmuramic acid kinase (ANMK) bound to adenosine diphosphate Pseudomonas aeruginosa 2.23 X-RAY DIFFRACTION 100.0
4MO5 TRANSFERASE 09/11/13 Crystal structure of AnmK bound to AMPPCP and anhMurNAc Pseudomonas aeruginosa 1.75 X-RAY DIFFRACTION 100.0
3QBX TRANSFERASE 01/14/11 Crystal structure of pseudomonas aeruginosa 1,6-anhydro-n-actetylmuramic acid kinase (ANMK) bound to 1,6-anhydro-n-actetylmuramic acid Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 319 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000645 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0666
Search term: anmK

Human Homologs

References

Molecular basis of 1,6-anhydro bond cleavage and phosphoryl transfer by Pseudomonas aeruginosa 1,6-anhydro-N-acetylmuramic acid kinase.
Bacik JP, Whitworth GE, Stubbs KA, Yadav AK, Martin DR, Bailey-Elkin BA, Vocadlo DJ, Mark BL
J Biol Chem 2011 Apr 8;286(14):12283-91
PubMed ID: 21288904