Pseudomonas aeruginosa PAO1, PA0797 (pmiR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0797
Name
pmiR
Replicon chromosome
Genomic location 875113 - 875835 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249488.1
GI 15595994
Affymetrix PA0797_at
DNASU PaCD00006361
Entrez 877945
GenBank AAG04186.1
INSDC AAG04186.1
NCBI Locus Tag PA0797
protein_id(GenBank) gb|AAG04186.1|AE004515_2|gnl|PseudoCAP|PA0797
TIGR NTL03PA00798
UniParc UPI00000C515A
UniProtKB Acc Q9I5E1
UniProtKB ID Q9I5E1_PSEAE
UniRef100 UniRef100_A0A069Q4N7
UniRef50 UniRef50_G8Q028
UniRef90 UniRef90_W0YMM0

Product

Feature Type CDS
Coding Frame 1
Product Name
PmiR
Product Name Confidence: Class 1
Synonyms pqs regulation and MIC receptor, PmiR
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.65
Kyte-Doolittle Hydrophobicity Value -0.504
Molecular Weight (kDa) 27.3
Isoelectric Point (pI) 6.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7XP1 SIGNALING PROTEIN 05/02/22 Crystal structure of PmiR from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.5 X-RAY DIFFRACTION 100.0
7XP0 SIGNALING PROTEIN 05/02/22 Crystal structure of PmiR from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.61 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 396 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000770 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0797
Search term: pmiR
Search term: PmiR

Human Homologs

References

Genetic instability in Streptomyces ambofaciens: inducibility and associated genome plasticity.
Simonet JM, Schneider D, Volff JN, Dary A, Decaris B
Gene 1992 Jun 15;115(1-2):49-54
PubMed ID: 1612449
PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa.
Cui G, Zhang Y, Xu X, Liu Y, Li Z, Wu M, Liu J, Gan J, Liang H
Sci Adv 2022 Dec 9;8(49):eadd4220
PubMed ID: 36475801