Pseudomonas aeruginosa PAO1, PA0903 (alaS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0903
Name
alaS
Synonym: sya
Replicon chromosome
Genomic location 986818 - 989442 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_249594.1
GI 15596100
Affymetrix PA0903_alaS_at
Entrez 878299
GenBank AAG04292.1
INSDC AAC46017.1
NCBI Locus Tag PA0903
protein_id(GenBank) gb|AAG04292.1|AE004524_9|gnl|PseudoCAP|PA0903
TIGR NTL03PA00904
UniParc UPI0000136317
UniProtKB Acc Q9I553
UniProtKB ID SYA_PSEAE
UniRef100 UniRef100_Q9I553
UniRef50 UniRef50_A7ZQC5
UniRef90 UniRef90_Q9I553

Product

Feature Type CDS
Coding Frame 1
Product Name
alanyl-tRNA synthetase
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -24.87
Kyte-Doolittle Hydrophobicity Value -0.256
Molecular Weight (kDa) 94.7
Isoelectric Point (pI) 5.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
aminocoumarin resistant alaS alanyl-tRNA synthetase conferring resistance to novobiocin in Escherichia coli ARO:3003830 protein homolog model Strict 0.0 0.66354 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000870 (559 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0903
Search term: alaS

Human Homologs

Ensembl 86, assembly GRCh38.p7
alanyl-tRNA synthetase [Source:HGNC Symbol;Acc:HGNC:20]
E-value: 5.0e-143
Percent Identity: 40.6

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 2840
Genomic Position 988832
Position in ORF 2015(2625)
Reference 14617778

Transposon Insertions in Orthologs

References

Evidence for a "cysteine-histidine box" metal-binding site in an Escherichia coli aminoacyl-tRNA synthetase.
Miller WT, Hill KA, Schimmel P
Biochemistry 1991 Jul;30(28):6970-6
PubMed ID: 1712632
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994