Pseudomonas aeruginosa PAO1, PA0920

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0920
Name
Replicon chromosome
Genomic location 1003966 - 1006611 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249611.1
GI 15596117
Affymetrix PA0920_at
Entrez 880933
GenBank AAG04309.1
INSDC AAG04309.1
NCBI Locus Tag PA0920
protein_id(GenBank) gb|AAG04309.1|AE004526_4|gnl|PseudoCAP|PA0920
TIGR NTL03PA00921
UniParc UPI00000C51BB
UniProtKB Acc Q9I537
UniProtKB ID Q9I537_PSEAE
UniRef100 UniRef100_Q9I537
UniRef50 UniRef50_A0A0B7D7G3
UniRef90 UniRef90_Q9I537

Product

Feature Type CDS
Coding Frame 1
Product Name
alanyl-phosphatidylglycerol synthase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 21.59
Kyte-Doolittle Hydrophobicity Value 0.484
Molecular Weight (kDa) 95.9
Isoelectric Point (pI) 9.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
Brucella suis mprF MprF is a integral membrane protein that modifies the negatively-charged phosphatidylglycerol on the membrane surface. This confers resistance to cationic peptides that disrupt the cell membrane, including defensins. ARO:3003772 protein homolog model Strict 0.0 0.389401 1.1.8

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4V34 TRANSFERASE 10/16/14 The Structure of A-PGS from Pseudomonas aeruginosa (SeMet derivative) PSEUDOMONAS AERUGINOSA 3.1 X-RAY DIFFRACTION 99.1
4V35 TRANSFERASE 10/16/14 The Structure of A-PGS from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 99.1

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 193 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000886 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0920

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 52046
Genomic Position 1004523
Position in ORF 558(2646)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 40039
Genomic Position 1005715
Position in ORF 1750(2646)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_20_G10
Genomic Position 1005413
Position in ORF 1448(2646)
Reference 15805499

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and sequencing of a 27.8-kb nucleotide sequence of the 79 degrees-81 degrees region of the Bacillus subtilis genome containing the sspE locus.
Yamamoto H, Uchiyama S, Sekiguchi J
DNA Res. 1996 Aug;3(4):257-62
PubMed ID: 8946165
Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa.
Hebecker S, Arendt W, Heinemann IU, Tiefenau JH, Nimtz M, Rohde M, Söll D, Moser J
Mol. Microbiol. 2011 May;80(4):935-50
PubMed ID: 21392131
Identification and characterization of a periplasmic aminoacyl-phosphatidylglycerol hydrolase responsible for Pseudomonas aeruginosa lipid homeostasis.
Arendt W, Groenewold MK, Hebecker S, Dickschat JS, Moser J
J. Biol. Chem. 2013 Aug;288(34):24717-30
PubMed ID: 23792962
Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine.
Hebecker S, Krausze J, Hasenkampf T, Schneider J, Groenewold M, Reichelt J, Jahn D, Heinz DW, Moser J
Proc. Natl. Acad. Sci. U.S.A. 2015 Aug;112(34):10691-6
PubMed ID: 26261323