Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0972
|
Name |
tolB
|
Replicon | chromosome |
Genomic location | 1056049 - 1057347 (+ strand) |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_249663.1 |
GI | 15596169 |
Affymetrix | PA0972_tolB_at |
Entrez | 881864 |
GenBank | AAG04361.1 |
INSDC | AAC44661.2 |
NCBI Locus Tag | PA0972 |
protein_id(GenBank) | gb|AAG04361.1|AE004530_14|gnl|PseudoCAP|PA0972 |
TIGR | NTL03PA00973 |
UniParc | UPI000013710C |
UniProtKB Acc | P50601 |
UniProtKB ID | TOLB_PSEAE |
UniRef100 | UniRef100_P50601 |
UniRef50 | UniRef50_Q4K7E4 |
UniRef90 | UniRef90_P50601 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
TolB protein
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (47.75 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | 3.48 |
Kyte-Doolittle Hydrophobicity Value | -0.266 |
Molecular Weight (kDa) | 47.7 |
Isoelectric Point (pI) | 9.23 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 375 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000937 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0972
Search term: tolB
Search term: TolB protein
|
Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli.
Levengood SK, Webster RE
J Bacteriol 1989 Dec;171(12):6600-9
PubMed ID: 2687247
|
Maturation and localization of the TolB protein required for colicin import.
Isnard M, Rigal A, Lazzaroni JC, Lazdunski C, Lloubes R
J Bacteriol 1994 Oct;176(20):6392-6
PubMed ID: 7929011
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|