Pseudomonas aeruginosa PAO1, PA1000 (pqsE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1000
Name
pqsE
Replicon chromosome
Genomic location 1082949 - 1083854 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249691.1
GI 15596197
Affymetrix PA1000_at
Entrez 880721
GenBank AAG04389.1
INSDC AAG04389.1
NCBI Locus Tag PA1000
protein_id(GenBank) gb|AAG04389.1|AE004532_13|gnl|PseudoCAP|PA1000
TIGR NTL03PA01001
UniParc UPI0000139EE3
UniProtKB Acc P20581
UniProtKB ID Y1000_PSEAE
UniRef100 UniRef100_P20581
UniRef50 UniRef50_P20581
UniRef90 UniRef90_P20581

Product

Feature Type CDS
Coding Frame 1
Product Name
Quinolone signal response protein
Product Name Confidence: Class 1
Commentrequired for phenazine, and cyanide production
Commentmay have a role in the cellular response to PQS
CommentQuinolone signal response
Commentnot required for Pseudomonas quinolone signal(PQS) synthesis
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.61
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 34.3
Isoelectric Point (pI) 5.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5HIO METAL BINDING PROTEIN 01/12/16 Crystal Structure of PQS Response Protein PqsE in Complex with 2-aminobenzoylacetate PSEUDOMONAS AERUGINOSA PAO1 1.9 X-RAY DIFFRACTION 100.0
5HIS METAL BINDING PROTEIN 01/12/16 Crystal Structure of PQS Response Protein PqsE in Complex with 3-methylthiophene-2-carboxylic acid PSEUDOMONAS AERUGINOSA PAO1 1.77 X-RAY DIFFRACTION 100.0
2Q0I METAL BINDING PROTEIN 05/22/07 Structure of Pseudomonas Quinolone Signal Response Protein PqsE PSEUDOMONAS AERUGINOSA 1.57 X-RAY DIFFRACTION 100.0
5HIQ METAL BINDING PROTEIN 01/12/16 Crystal Structure of PQS Response Protein PqsE in Complex with 2-(1H-pyrrol-1-yl)benzoic acid PSEUDOMONAS AERUGINOSA PAO1 2.1 X-RAY DIFFRACTION 100.0
5HIP METAL BINDING PROTEIN 01/12/16 Crystal Structure of PQS Response Protein PqsE in Complex with 2-(pyridin-3-yl)benzoic acid PSEUDOMONAS AERUGINOSA PAO1 1.992 X-RAY DIFFRACTION 100.0
2VW8 SIGNALING PROTEIN 06/17/08 Crystal Structure of Quinolone signal response protein pqsE from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 1.45 X-RAY DIFFRACTION 100.0
3DH8 METAL BINDING PROTEIN 06/17/08 Structure of Pseudomonas Quinolone Signal Response Protein PqsE PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 99.7
2Q0J METAL BINDING PROTEIN 05/22/07 Structure of Pseudomonas Quinolone Signal Response Protein PqsE PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
bis(4-nitrophenyl) hydrogen phosphate DB07418 DrugBank 5.0.6 P20581 Uncharacterized protein PA1000 100.0 301 1.3e-176

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 100 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000963 (302 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1000
Search term: pqsE

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 17750
Genomic Position 1083757
Position in ORF 809(906)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_76_C11
Genomic Position 1083381
Position in ORF 433(906)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 1308
Genomic Position 1083267
Position in ORF 319(906)
Reference 14617778

Transposon Insertions in Orthologs

References

Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications.
Essar DW, Eberly L, Hadero A, Crawford IP
J. Bacteriol. 1990 Feb;172(2):884-900
PubMed ID: 2153661