Pseudomonas aeruginosa PAO1, PA1148 (toxA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1148
Name
toxA
Synonym: eta
Replicon chromosome
Genomic location 1240584 - 1242500 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249839.1
GI 15596345
Affymetrix PA1148_toxA_at
Entrez 877850
GenBank AAG04537.1
INSDC AAG04537.1
NCBI Locus Tag PA1148
protein_id(GenBank) gb|AAG04537.1|AE004544_9|gnl|PseudoCAP|PA1148
TIGR NTL03PA01149
UniParc UPI0000137188
UniProtKB Acc P11439
UniProtKB ID TOXA_PSEAE
UniRef100 UniRef100_P11439
UniRef50 UniRef50_P11439
UniRef90 UniRef90_P11439

Product

Feature Type CDS
Coding Frame 1
Product Name
exotoxin A precursor
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -16.12
Kyte-Doolittle Hydrophobicity Value -0.262
Molecular Weight (kDa) 69.3
Isoelectric Point (pI) 5.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1IKP TRANSFERASE 05/04/01 Pseudomonas Aeruginosa Exotoxin A, P201Q, W281A mutant PSEUDOMONAS AERUGINOSA 1.45 X-RAY DIFFRACTION 99.2
1DMA ADP-RIBOSYLATION 04/28/95 DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH NICOTINAMIDE AND AMP PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 99.1
1XK9 TRANSFERASE 09/28/04 Pseudomanas exotoxin A in complex with the PJ34 inhibitor PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 99.1
1ZM9 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of eEF2-ETA in complex with PJ34 PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 2.8 X-RAY DIFFRACTION 99.0
1IKQ TRANSFERASE 05/04/01 Pseudomonas Aeruginosa Exotoxin A, wild type PSEUDOMONAS AERUGINOSA 1.62 X-RAY DIFFRACTION 99.5
1ZM4 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of the eEF2-ETA-bTAD complex PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 2.9 X-RAY DIFFRACTION 99.0
2ZIT BIOSYNTHETIC PROTEIN/TRANSFERASE 02/24/08 Structure of the eEF2-ExoA-NAD+ complex PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 3.0 X-RAY DIFFRACTION 99.0
1ZM3 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of the apo eEF2-ETA complex PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 3.07 X-RAY DIFFRACTION 99.0
1ZM2 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 3.07 X-RAY DIFFRACTION 99.0
1AER ADP-RIBOSYLATION 12/11/95 DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH BETA-TAD PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 99.0
3B78 BIOSYNTHETIC PROTEIN/TRANSFERASE 10/30/07 Structure of the eEF2-ExoA(R551H)-NAD+ complex PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 2.5 X-RAY DIFFRACTION 98.5

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Nicotinamide DB02701 DrugBank 5.0.6 P11439 Exotoxin A 100.0 638 0.0e+00
N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE DB08348 DrugBank 5.0.6 P11439 Exotoxin A 100.0 638 0.0e+00

Virulence Evidence

Source 1
Database VFDB : VF0086
Category Exototoxin A
Evidence
Host Organism
Notes
Reference 12270928
Source 2
Database Victors : 4206
Category Exototoxin A
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes toxA (exotoxin A) blocks protein synthesis by ADP ribosylation of elongation factor 2, thereby triggering cell death
Reference 19380469

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001108 (299 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1148
Search term: toxA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 43788
Genomic Position 1242284
Position in ORF 1701(1917)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 40695
Genomic Position 1241834
Position in ORF 1251(1917)
Reference 14617778

Transposon Insertions in Orthologs

References

Mechanism of protein excretion by gram-negative bacteria: Pseudomonas aeruginosa exotoxin A.
Lory S, Tai PC, Davis BD
J. Bacteriol. 1983 Nov;156(2):695-702
PubMed ID: 6415037
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning, nucleotide sequence, and expression in Escherichia coli of the exotoxin A structural gene of Pseudomonas aeruginosa.
Gray GL, Smith DH, Baldridge JS, Harkins RN, Vasil ML, Chen EY, Heyneker HL
Proc. Natl. Acad. Sci. U.S.A. 1984 May;81(9):2645-9
PubMed ID: 6201861