Pseudomonas aeruginosa PAO1, PA1171 (sltB2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1171
Name
sltB2
Synonym: soluble lytic transglycosylase
Replicon chromosome
Genomic location 1270972 - 1272168 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249862.1
GI 15596368
Affymetrix PA1171_at
Entrez 880763
GenBank AAG04560.1
INSDC AAG04560.1
NCBI Locus Tag PA1171
protein_id(GenBank) gb|AAG04560.1|AE004547_6|gnl|PseudoCAP|PA1171
TIGR NTL03PA01172
UniParc UPI00000C5289
UniProtKB Acc Q9I4G6
UniProtKB ID Q9I4G6_PSEAE
UniRef100 UniRef100_A0A0C7D5V3
UniRef50 UniRef50_A0A011NW55
UniRef90 UniRef90_B7UX18

Product

Feature Type CDS
Coding Frame 1
Product Name
SltB2
Product Name Confidence: Class 2
Synonyms probable transglycolase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 5.42
Kyte-Doolittle Hydrophobicity Value -0.169
Molecular Weight (kDa) 42.7
Isoelectric Point (pI) 9.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7QVD HYDROLASE 01/21/22 X-ray structure of the lytic transglycosylase SltB2 from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 221 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001129 (305 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1171
Search term: sltB2
Search term: SltB2

Human Homologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.
Blackburn NT, Clarke AJ
Biochemistry 2002 Jan 22;41(3):1001-13
PubMed ID: 11790124