Pseudomonas aeruginosa PAO1, PA1282

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1282
Name
Replicon chromosome
Genomic location 1392564 - 1394069 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249973.1
GI 15596479
Affymetrix PA1282_at
Entrez 881440
GenBank AAG04671.1
INSDC AAG04671.1
NCBI Locus Tag PA1282
protein_id(GenBank) gb|AAG04671.1|AE004557_12|gnl|PseudoCAP|PA1282
TIGR NTL03PA01283
UniParc UPI00000C52EA
UniProtKB Acc Q9I462
UniProtKB ID Q9I462_PSEAE
UniRef100 UniRef100_Q9I462
UniRef50 UniRef50_Q6CYP9
UniRef90 UniRef90_Q9I462

Product

Feature Type CDS
Coding Frame 1
Product Name
probable major facilitator superfamily (MFS) transporter
Product Name Confidence: Class 3
Evidence for Translation
Charge (pH 7) 7.08
Kyte-Doolittle Hydrophobicity Value 0.840
Molecular Weight (kDa) 52.6
Isoelectric Point (pI) 9.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
lrfA lfrA is involved in the active efflux of quinolones and is found in Mycobacterium smegmatis. ARO:3003967 protein homolog model Strict 0.0 0.385809 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 503 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001213 (1431 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1282

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 47004
Genomic Position 1393569
Position in ORF 1006(1506)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 4790
Genomic Position 1393085
Position in ORF 522(1506)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor.
Caballero JL, Martinez E, Malpartida F, Hopwood DA
Mol. Gen. Genet. 1991 Dec;230(3):401-12
PubMed ID: 1766437
Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis.
Takiff HE, Cimino M, Musso MC, Weisbrod T, Martinez R, Delgado MB, Salazar L, Bloom BR, Jacobs WR
Proc. Natl. Acad. Sci. U.S.A. 1996 Jan;93(1):362-6
PubMed ID: 8552639
Multidrug resistance proteins QacA and QacB from Staphylococcus aureus: membrane topology and identification of residues involved in substrate specificity.
Paulsen IT, Brown MH, Littlejohn TG, Mitchell BA, Skurray RA
Proc. Natl. Acad. Sci. U.S.A. 1996 Apr;93(8):3630-5
PubMed ID: 8622987