Pseudomonas aeruginosa PAO1, PA1304

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1304
Name
Replicon chromosome
Genomic location 1414763 - 1416814 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 2XE4: oligopeptidase B from Leishmania major (root-mean-square deviation: 0.6 angstroms)

Cross-References

RefSeq NP_249995.1
GI 15596501
Affymetrix PA1304_at
Entrez 881476
GenBank AAG04693.1
INSDC AAG04693.1
NCBI Locus Tag PA1304
protein_id(GenBank) gb|AAG04693.1|AE004560_1|gnl|PseudoCAP|PA1304
TIGR NTL03PA01305
UniParc UPI00000C52FF
UniProtKB Acc Q9I440
UniProtKB ID Q9I440_PSEAE
UniRef100 UniRef100_S0HYL3
UniRef50 UniRef50_Q4KGJ9
UniRef90 UniRef90_A6V8Q6

Product

Feature Type CDS
Coding Frame 1
Product Name
probable oligopeptidase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -29.84
Kyte-Doolittle Hydrophobicity Value -0.347
Molecular Weight (kDa) 75.9
Isoelectric Point (pI) 4.70

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 323 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001247 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1304

Human Homologs

Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8
Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8
Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8
Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8
Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8
Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8
Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8
Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8
Ensembl 110, assembly GRCh38.p14
prolyl endopeptidase like [Source:HGNC Symbol;Acc:HGNC:30228]
E-value: 2.5e-54
Percent Identity: 28.8

References

The gene encoding dipeptidyl aminopeptidase BI from Pseudomonas sp. WO24: cloning, sequencing and expression in Escherichia coli.
Ogasawara W, Kobayashi G, Ishimaru S, Okada H, Morikawa Y
Gene 1998 Jan 12;206(2):229-36
PubMed ID: 9469937
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799