Pseudomonas aeruginosa PAO1, PA1435

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1435
Name
Replicon chromosome
Genomic location 1562813 - 1563970 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_250126.1
GI 15596632
Affymetrix PA1435_at
Entrez 881806
GenBank AAG04824.1
INSDC AAG04824.1
NCBI Locus Tag PA1435
protein_id(GenBank) gb|AAG04824.1|AE004573_3|gnl|PseudoCAP|PA1435
TIGR NTL03PA01436
UniParc UPI00000C537D
UniProtKB Acc Q9I3R2
UniProtKB ID Q9I3R2_PSEAE
UniRef100 UniRef100_Q4LDT9
UniRef50 UniRef50_Q0K9R0
UniRef90 UniRef90_B7UWB7

Product

Feature Type CDS
Coding Frame 1
Product Name
probable Resistance-Nodulation-Cell Division (RND) efflux membrane fusion protein precursor
Product Name Confidence: Class 3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.73
Kyte-Doolittle Hydrophobicity Value -0.049
Molecular Weight (kDa) 41.2
Isoelectric Point (pI) 9.76

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
mexM mexM is the membrane fusion protein of the MexMN-OprM multidrug efflux complex. ARO:3003704 protein homolog model Perfect 0.0 1.0 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 417 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001352 (474 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1435

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 5365
Genomic Position 1563388
Position in ORF 576(1158)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 11360
Genomic Position 1563429
Position in ORF 617(1158)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295