Pseudomonas aeruginosa PAO1, PA1493 (cysP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1493
Name
cysP
Replicon chromosome
Genomic location 1620345 - 1621343 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250184.1
GI 15596690
Affymetrix PA1493_cysP_at
Entrez 881623
GenBank AAG04882.1
INSDC AAG04882.1
NCBI Locus Tag PA1493
protein_id(GenBank) gb|AAG04882.1|AE004578_4|gnl|PseudoCAP|PA1493
TIGR NTL03PA01494
UniParc UPI00000C53A7
UniProtKB Acc Q9I3L9
UniProtKB ID Q9I3L9_PSEAE
UniRef100 UniRef100_W0YM00
UniRef50 UniRef50_P0AG78
UniRef90 UniRef90_A6V808

Product

Feature Type CDS
Coding Frame 1
Product Name
sulfate-binding protein of ABC transporter
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 1.72
Kyte-Doolittle Hydrophobicity Value -0.325
Molecular Weight (kDa) 36.5
Isoelectric Point (pI) 8.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 212 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001407 (498 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1493
Search term: cysP

Human Homologs

References

Amino acid sequence of the sulfate-binding protein from Salmonella typhimurium LT2.
Isihara H, Hogg RW
J Biol Chem 1980 May 25;255(10):4614-8
PubMed ID: 6989815
Engineered interdomain disulfide in the periplasmic receptor for sulfate transport reduces flexibility. Site-directed mutagenesis and ligand-binding studies.
Jacobson BL, He JJ, Vermersch PS, Lemon DD, Quiocho FA
J Biol Chem 1991 Mar 15;266(8):5220-5
PubMed ID: 2002055
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299