Pseudomonas aeruginosa PAO1, PA1587 (lpd)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1587
Name
lpd
Synonym: lpdA
Replicon chromosome
Genomic location 1728416 - 1729852 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_250278.1
GI 15596784
Affymetrix PA1587_lpdG_at
Entrez 882090
GenBank AAG04976.1
INSDC AAG04976.1
NCBI Locus Tag PA1587
protein_id(GenBank) gb|AAG04976.1|AE004586_14|gnl|PseudoCAP|PA1587
TIGR NTL03PA01588
UniParc UPI00000C53F6
UniProtKB Acc Q9I3D1
UniProtKB ID DLDH2_PSEAE
UniRef100 UniRef100_Q9I3D1
UniRef50 UniRef50_P14218
UniRef90 UniRef90_P14218

Product

Feature Type CDS
Coding Frame 1
Product Name
dihydrolipoamide dehydrogenase Lpd
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (50.16 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.22
Kyte-Doolittle Hydrophobicity Value 0.135
Molecular Weight (kDa) 50.2
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Outer Membrane Class 1
Cytoplasmic Class 2
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5U8V OXIDOREDUCTASE 12/15/16 Dihydrolipoamide dehydrogenase (LpdG) from Pseudomonas aeruginosa bound to NAD+ PSEUDOMONAS AERUGINOSA (STRAIN UCBPP-PA14) 1.45 X-RAY DIFFRACTION 100.0
1LPF OXIDOREDUCTASE 10/26/92 THREE-DIMENSIONAL STRUCTURE OF LIPOAMIDE DEHYDROGENASE FROM PSEUDOMONAS FLUORESCENS AT 2.8 ANGSTROMS RESOLUTION. ANALYSIS OF REDOX AND THERMOSTABILITY PROPERTIES PSEUDOMONAS FLUORESCENS 2.8 X-RAY DIFFRACTION 99.8
5U8U OXIDOREDUCTASE 12/15/16 Dihydrolipoamide dehydrogenase (LpdG) from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN UCBPP-PA14) 1.35 X-RAY DIFFRACTION 100.0
5U8W OXIDOREDUCTASE 12/15/16 Dihydrolipoamide dehydrogenase (LpdG) from Pseudomonas aeruginosa bound to NADH PSEUDOMONAS AERUGINOSA (STRAIN UCBPP-PA14) 1.79 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Flavin adenine dinucleotide DB03147 DrugBank 5.0.6 P14218 Dihydrolipoyl dehydrogenase 99.8 478 1.1e-267

Virulence Evidence

Source 1
Database PseudoCAP
Category dihydrolipoamide dehydrogenase
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Homo sapiens
Notes
Infection model Serum bactericidal assay
Reference 23071278

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 650 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003776 (563 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1587
Search term: lpd

Human Homologs

Ensembl 86, assembly GRCh38.p7
dihydrolipoamide dehydrogenase [Source:HGNC Symbol;Acc:HGNC:2898]
E-value: 1.2e-122
Percent Identity: 49.8

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 36716
Genomic Position 1729735
Position in ORF 1320(1437)
Reference 14617778

Transposon Insertions in Orthologs

References

Sequence analysis of the lpdV gene for lipoamide dehydrogenase of branched-chain-oxoacid dehydrogenase of Pseudomonas putida.
Burns G, Brown T, Hatter K, Sokatch JR
Eur. J. Biochem. 1989 Jan;179(1):61-9
PubMed ID: 2917566
Cloning and sequence analysis of the LPD-glc structural gene of Pseudomonas putida.
Palmer JA, Hatter K, Sokatch JR
J. Bacteriol. 1991 May;173(10):3109-16
PubMed ID: 1902462
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Dihydrolipoamide dehydrogenase of Pseudomonas aeruginosa is a surface-exposed immune evasion protein that binds three members of the factor H family and plasminogen.
Hallström T, Mörgelin M, Barthel D, Raguse M, Kunert A, Hoffmann R, Skerka C, Zipfel PF
J. Immunol. 2012 Nov;189(10):4939-50
PubMed ID: 23071278