Pseudomonas aeruginosa PAO1, PA1754 (cysB)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 1894249 - 1895223 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_250445.1
GI 15596951
Affymetrix PA1754_cysB_at
DNASU PaCD00006799
Entrez 883007
GenBank AAG05143.1
INSDC AAG05143.1
NCBI Locus Tag PA1754
protein_id(GenBank) gb|AAG05143.1|AE004601_8|gnl|PseudoCAP|PA1754
UniParc UPI00000D4219
UniProtKB Acc G3XCU6
UniRef100 UniRef100_W0YS31
UniRef50 UniRef50_P06614
UniRef90 UniRef90_Q4K8S3


Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator CysB
Product Name Confidence: Class 1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.01
Kyte-Doolittle Hydrophobicity Value 0.019
Molecular Weight (kDa) 36.1
Isoelectric Point (pI) 7.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
aminocoumarin resistant cysB Positive regulator of gene expression in the cysteine regulon. cysB mutants confer resistance to novobiocin, an aminocoumarin antibiotic, in Escherichia coli. ARO:3003840 protein homolog model Strict 0.0 0.636646 1.1.8

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 535 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003639 (561 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1754
Search term: cysB


Cloning, sequence and mutagenesis of the structural gene of Pseudomonas aeruginosa CysB, which can activate algD transcription.
Delic-Attree I, Toussaint B, Garin J, Vignais PM
Mol. Microbiol. 1997 Jun;24(6):1275-84
PubMed ID: 9218775
Transcriptional control of the pvdS iron starvation sigma factor gene by the master regulator of sulfur metabolism CysB in Pseudomonas aeruginosa.
Imperi F, Tiburzi F, Fimia GM, Visca P
Environ. Microbiol. 2010 Jun;12(6):1630-42
PubMed ID: 20370820
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994