Pseudomonas aeruginosa PAO1, PA1754 (cysB)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 1894249 - 1895223 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_250445.1
GI 15596951
Affymetrix PA1754_cysB_at
DNASU PaCD00006799
Entrez 883007
GenBank AAG05143.1
INSDC AAG05143.1
NCBI Locus Tag PA1754
protein_id(GenBank) gb|AAG05143.1|AE004601_8|gnl|PseudoCAP|PA1754
UniParc UPI00000D4219
UniProtKB Acc G3XCU6
UniRef100 UniRef100_W0YS31
UniRef50 UniRef50_P06614
UniRef90 UniRef90_Q4K8S3


Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator CysB
Product Name Confidence: Class 1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.01
Kyte-Doolittle Hydrophobicity Value 0.019
Molecular Weight (kDa) 36.1
Isoelectric Point (pI) 7.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
aminocoumarin resistant cysB Positive regulator of gene expression in the cysteine regulon. cysB mutants confer resistance to novobiocin, an aminocoumarin antibiotic, in Escherichia coli. ARO:3003840 protein homolog model Strict 0.0 0.636646 1.1.8

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 535 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003639 (561 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1754
Search term: cysB

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 19415
Genomic Position 1894925
Position in ORF 677(975)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 1812
Genomic Position 1894885
Position in ORF 637(975)
Reference 14617778

Transposon Insertions in Orthologs


Cloning, sequence and mutagenesis of the structural gene of Pseudomonas aeruginosa CysB, which can activate algD transcription.
Delic-Attree I, Toussaint B, Garin J, Vignais PM
Mol. Microbiol. 1997 Jun;24(6):1275-84
PubMed ID: 9218775
Transcriptional control of the pvdS iron starvation sigma factor gene by the master regulator of sulfur metabolism CysB in Pseudomonas aeruginosa.
Imperi F, Tiburzi F, Fimia GM, Visca P
Environ. Microbiol. 2010 Jun;12(6):1630-42
PubMed ID: 20370820
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994