Pseudomonas aeruginosa PAO1, PA1982 (exaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1982
Name
exaA
Replicon chromosome
Genomic location 2167281 - 2169152 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_250672.1
GI 15597178
Affymetrix PA1982_exaA_at
Entrez 880475
GenBank AAG05370.1
INSDC AAC79657.1
NCBI Locus Tag PA1982
protein_id(GenBank) gb|AAG05370.1|AE004624_9|gnl|PseudoCAP|PA1982
TIGR NTL03PA01982
UniParc UPI000012A32B
UniProtKB Acc Q9Z4J7
UniProtKB ID EXAA_PSEAE
UniRef100 UniRef100_Q9Z4J7
UniRef50 UniRef50_A8R3S4
UniRef90 UniRef90_Q9Z4J7

Product

Feature Type CDS
Coding Frame 1
Product Name
quinoprotein ethanol dehydrogenase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:6.6, molecular weight:68.1, PMID:21674802)
Charge (pH 7) 0.77
Kyte-Doolittle Hydrophobicity Value -0.463
Molecular Weight (kDa) 68.1
Isoelectric Point (pI) 7.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1FLG OXIDOREDUCTASE 08/14/00 CRYSTAL STRUCTURE OF THE QUINOPROTEIN ETHANOL DEHYDROGENASE FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.6 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Pyrroloquinoline Quinone DB03205 DrugBank 5.0.6 Q9Z4J7 Quinoprotein ethanol dehydrogenase 100.0 623 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 163 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003450 (446 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1982
Search term: exaA

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_100_B3
Genomic Position 2168919
Position in ORF 1639(1872)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 8107
Genomic Position 2168137
Position in ORF 857(1872)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 47476
Genomic Position 2167768
Position in ORF 488(1872)
Reference 14617778

Transposon Insertions in Orthologs

References

Quaternary structure of quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa and its reoxidation with a novel cytochrome c from this organism.
Schrover JM, Frank J, van Wielink JE, Duine JA
Biochem. J. 1993 Feb;290 ( Pt 1)
PubMed ID: 8382472
Characterization of the gene encoding quinohaemoprotein ethanol dehydrogenase of Comamonas testosteroni.
Stoorvogel J, Kraayveld DE, Van Sluis CA, Jongejan JA, De Vries S, Duine JA
Eur. J. Biochem. 1996 Feb;235(3):690-8
PubMed ID: 8654419
Quinoprotein ethanol dehydrogenase of Pseudomonas aeruginosa is a homodimer--sequence of the gene and deduced structural properties of the enzyme.
Diehl A, von Wintzingerode F, Görisch H
Eur. J. Biochem. 1998 Oct;257(2):409-19
PubMed ID: 9826187
A complex regulatory network controls aerobic ethanol oxidation in Pseudomonas aeruginosa: indication of four levels of sensor kinases and response regulators.
Mern DS, Ha SW, Khodaverdi V, Gliese N, Görisch H
Microbiology (Reading, Engl.) 2010 May;156(Pt 5):1505-16
PubMed ID: 20093290
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802