Pseudomonas aeruginosa PAO1, PA2018 (mexY)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2018
Name
mexY
Synonym: mexH
Synonym: amrB
Replicon chromosome
Genomic location 2208169 - 2211306 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_250708.1
GI 15597214
Affymetrix PA2018_at
Entrez 878882
GenBank AAG05406.1
INSDC AAG05406.1
NCBI Locus Tag PA2018
protein_id(GenBank) gb|AAG05406.1|AE004628_4|gnl|PseudoCAP|PA2018
TIGR NTL03PA02018
UniParc UPI00000D420B
UniProtKB Acc G3XCW2
UniProtKB ID G3XCW2_PSEAE
UniRef100 UniRef100_G3XCW2
UniRef50 UniRef50_P31224
UniRef90 UniRef90_G3XCW2

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexY
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.58
Kyte-Doolittle Hydrophobicity Value 0.341
Molecular Weight (kDa) 112.7
Isoelectric Point (pI) 6.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
amrB amrB is the membrane fusion protein of the AmrAB-OprM multidrug efflux complex. ARO:3002983 protein homolog model Perfect 0.0 1.0 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000417 (2173 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2018
Search term: mexY

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 31612
Genomic Position 2209584
Position in ORF 1416(3138)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 29914
Genomic Position 2210166
Position in ORF 1998(3138)
Reference 14617778

Transposon Insertions in Orthologs

References

Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei.
Moore RA, DeShazer D, Reckseidler S, Weissman A, Woods DE
Antimicrob. Agents Chemother. 1999 Mar;43(3):465-70
PubMed ID: 10049252
MexXY multidrug efflux system of Pseudomonas aeruginosa.
Morita Y, Tomida J, Kawamura Y
Front Microbiol 2012 ;3
PubMed ID: 23233851
Involvement of an active efflux system in the natural resistance of Pseudomonas aeruginosa to aminoglycosides.
Aires JR, Köhler T, Nikaido H, Plésiat P
Antimicrob. Agents Chemother. 1999 Nov;43(11):2624-8
PubMed ID: 10543738
Contribution of the MexX-MexY-oprM efflux system to intrinsic resistance in Pseudomonas aeruginosa.
Masuda N, Sakagawa E, Ohya S, Gotoh N, Tsujimoto H, Nishino T
Antimicrob. Agents Chemother. 2000 Sep;44(9):2242-6
PubMed ID: 10952562
Induction of the MexXY efflux pump in Pseudomonas aeruginosa is dependent on drug-ribosome interaction.
Jeannot K, Sobel ML, El Garch F, Poole K, Plésiat P
J. Bacteriol. 2005 Aug;187(15):5341-6
PubMed ID: 16030228