Pseudomonas aeruginosa PAO1, PA2019 (mexX)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2019
Name
mexX
Synonym: mexG
Synonym: amrA
Replicon chromosome
Genomic location 2211322 - 2212512 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_250709.1
GI 15597215
Affymetrix PA2019_at
Entrez 878839
GenBank AAG05407.1
INSDC AAG05407.1
NCBI Locus Tag PA2019
protein_id(GenBank) gb|AAG05407.1|AE004628_5|gnl|PseudoCAP|PA2019
TIGR NTL03PA02019
UniParc UPI00000D4247
UniProtKB Acc G3XD21
UniProtKB ID G3XD21_PSEAE
UniRef100 UniRef100_G3XD21
UniRef50 UniRef50_G3XD21
UniRef90 UniRef90_G3XD21

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux membrane fusion protein MexX precursor
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.05
Kyte-Doolittle Hydrophobicity Value -0.059
Molecular Weight (kDa) 42.1
Isoelectric Point (pI) 6.19

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
amrA amrA is the efflux pump subunit of the AmrAB-OprM multidrug efflux complex. ARO:3002982 protein homolog model Perfect 0.0 1.0 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 376 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003421 (317 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2019
Search term: mexX

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 7189
Genomic Position 2212203
Position in ORF 882(1191)
Reference 14617778

Transposon Insertions in Orthologs

References

Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei.
Moore RA, DeShazer D, Reckseidler S, Weissman A, Woods DE
Antimicrob. Agents Chemother. 1999 Mar;43(3):465-70
PubMed ID: 10049252
MexXY multidrug efflux system of Pseudomonas aeruginosa.
Morita Y, Tomida J, Kawamura Y
Front Microbiol 2012 ;3
PubMed ID: 23233851
Induction of the MexXY efflux pump in Pseudomonas aeruginosa is dependent on drug-ribosome interaction.
Jeannot K, Sobel ML, El Garch F, Poole K, Plésiat P
J. Bacteriol. 2005 Aug;187(15):5341-6
PubMed ID: 16030228