Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA2385
|
Name |
pvdQ
|
Replicon | chromosome |
Genomic location | 2636517 - 2638805 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Reaction described in reference. |
Comment | Encodes an enzyme with a homoserine lactone-releasing acyl-homoserine lactone (AHL) acylase activity specific towards AHLs |
RefSeq | NP_251075.1 |
GI | 15597581 |
Affymetrix | PA2385_at |
Entrez | 882260 |
GenBank | AAG05773.1 |
INSDC | AAG05773.1 |
NCBI Locus Tag | PA2385 |
protein_id(GenBank) | gb|AAG05773.1|AE004664_10|gnl|PseudoCAP|PA2385 |
TIGR | NTL03PA02385 |
UniParc | UPI00000C56A5 |
UniProtKB Acc | Q9I194 |
UniProtKB ID | PVDQ_PSEAE |
UniRef100 | UniRef100_Q9I194 |
UniRef50 | UniRef50_Q9I194 |
UniRef90 | UniRef90_Q9I194 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
3-oxo-C12-homoserine lactone acylase PvdQ
|
Synonyms |
PvdQ |
Evidence for Translation | |
Charge (pH 7) | 0.50 |
Kyte-Doolittle Hydrophobicity Value | -0.345 |
Molecular Weight (kDa) | 84.0 |
Isoelectric Point (pI) | 7.10 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4WKS | HYDROLASE/HYDROLASE INHIBITOR | 10/03/14 | n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ | Pseudomonas aeruginosa | 1.629 | X-RAY DIFFRACTION | 100.0 |
4WKU | HYDROLASE/HYDROLASE Inhibitor | 10/03/14 | n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
3SRC | HYDROLASE/HYDROLASE INHIBITOR | 07/07/11 | Structure of Pseudomonas aeruginosa PvdQ bound to NS2028 | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
4M1J | HYDROLASE | 08/02/13 | Crystal structure of Pseudomonas aeruginosa PvdQ in complex with a transition state analogue | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
5UBK | HYDROLASE | 12/20/16 | Inactive S1A/N269D-cpPvdQ mutant in complex with the pyoverdine precursor PVDIq reveals a specific binding pocket for the D-Tyr of this substrate | Pseudomonas aeruginosa | 2.55 | X-RAY DIFFRACTION | 99.6 |
3SRB | HYDROLASE/HYDROLASE INHIBITOR | 07/07/11 | Structure of Pseudomonas aeruginosa PvdQ bound to SMER28 | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
4K2F | HYDROLASE/HYDROLASE INHIBITOR | 04/09/13 | Structure of Pseudomonas aeruginosa PvdQ bound to BRD-A08522488 | Pseudomonas aeruginosa | 1.99 | X-RAY DIFFRACTION | 100.0 |
3SRA | HYDROLASE | 07/07/11 | Structure of Pseudomonas aerugionsa PvdQ covalently acylated with myristic acid from PVDIq | Pseudomonas aeruginosa PAO1 | 2.3 | X-RAY DIFFRACTION | 100.0 |
2WYB | HYDROLASE | 11/16/09 | The quorum quenching N-acyl homoserine lactone acylase PvdQ with a covalently bound dodecanoic acid | PSEUDOMONAS AERUGINOSA | 2.1 | X-RAY DIFFRACTION | 100.0 |
2WYE | HYDROLASE | 11/16/09 | The quorum quenching N-acyl homoserine lactone acylase PvdQ is an Ntn- Hydrolase with an unusual substrate-binding pocket | PSEUDOMONAS AERUGINOSA | 1.8 | X-RAY DIFFRACTION | 100.0 |
4BTH | HYDROLASE | 06/18/13 | The LeuA146Trp,PheB24Tyr Double Mutant of the Quorum Quenching N-acyl Homoserine Lactone Acylase PvdQ Has an Altered Substrate Specificity Towards Small Acyl Chains | PSEUDOMONAS AERUGINOSA | 1.9 | X-RAY DIFFRACTION | 99.8 |
5UBL | HYDROLASE | 12/20/16 | A circularly permuted version of PvdQ (cpPvdQ) | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
3L91 | HYDROLASE | 01/04/10 | Structure of Pseudomonas aerugionsa PvdQ bound to octanoate | Pseudomonas aeruginosa | 1.66 | X-RAY DIFFRACTION | 100.0 |
4K2G | HYDROLASE/HYDROLASE INHIBITOR | 04/09/13 | Structure of Pseudomonas aeruginosa PvdQ bound to BRD-A33442372 | Pseudomonas aeruginosa | 2.3 | X-RAY DIFFRACTION | 100.0 |
2WYC | HYDROLASE | 11/16/09 | The quorum quenching N-acyl homoserine lactone acylase PvdQ in complex with 3-oxo-lauric acid | PSEUDOMONAS AERUGINOSA | 1.9 | X-RAY DIFFRACTION | 100.0 |
3L94 | HYDROLASE | 01/04/10 | Structure of PvdQ covalently acylated with myristate | Pseudomonas aeruginosa | 1.95 | X-RAY DIFFRACTION | 100.0 |
4WKT | HYDROLASE/HYDROLASE Inhibitor | 10/03/14 | n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ | Pseudomonas aeruginosa | 1.782 | X-RAY DIFFRACTION | 100.0 |
2WYD | HYDROLASE | 11/16/09 | The quorum quenching N-acyl homoserine lactone acylase PvdQ in complex with dodecanoic acid | PSEUDOMONAS AERUGINOSA | 1.901 | X-RAY DIFFRACTION | 100.0 |
4WKV | HYDROLASE/HYDROLASE Inhibitor | 10/03/14 | n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ | Pseudomonas aeruginosa | 2.1434 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Lauric acid | DB03017 | DrugBank | 5.1.4 | Q9I194 | Acyl-homoserine lactone acylase PvdQ | 100.0 | 762 | 0.0e+00 |
Source 1 | |
Database | VFDB |
Category | Pyoverdine |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 180 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003142 (466 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA2385
Search term: pvdQ
Search term: 3-oxo-C12-homoserine lactone acylase PvdQ
|
Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa.
Lamont IL, Martin LW
Microbiology (Reading) 2003 Apr;149(Pt 4):833-842
PubMed ID: 12686626
|
Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1.
Sio CF, Otten LG, Cool RH, Diggle SP, Braun PG, Bos R, Daykin M, Cámara M, Williams P, Quax WJ
Infect Immun 2006 Mar;74(3):1673-82
PubMed ID: 16495538
|
The quorum-quenching N-acyl homoserine lactone acylase PvdQ is an Ntn-hydrolase with an unusual substrate-binding pocket.
Bokhove M, Nadal Jimenez P, Quax WJ, Dijkstra BW
Proc Natl Acad Sci U S A 2010 Jan 12;107(2):686-91
PubMed ID: 20080736
|