Pseudomonas aeruginosa PAO1, PA2385 (pvdQ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2385
Name
pvdQ
Replicon chromosome
Genomic location 2636517 - 2638805 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251075.1
GI 15597581
Affymetrix PA2385_at
Entrez 882260
GenBank AAG05773.1
INSDC AAG05773.1
NCBI Locus Tag PA2385
protein_id(GenBank) gb|AAG05773.1|AE004664_10|gnl|PseudoCAP|PA2385
TIGR NTL03PA02385
UniParc UPI00000C56A5
UniProtKB Acc Q9I194
UniProtKB ID PVDQ_PSEAE
UniRef100 UniRef100_Q9I194
UniRef50 UniRef50_Q9I194
UniRef90 UniRef90_Q9I194

Product

Feature Type CDS
Coding Frame 1
Product Name
3-oxo-C12-homoserine lactone acylase PvdQ
Product Name Confidence: Class 1
CommentEncodes an enzyme with a homoserine lactone-releasing acyl-homoserine lactone (AHL) acylase activity specific towards AHLs
CommentReaction described in reference.
Evidence for Translation
Charge (pH 7) 0.50
Kyte-Doolittle Hydrophobicity Value -0.345
Molecular Weight (kDa) 84.0
Isoelectric Point (pI) 7.10

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4BTH HYDROLASE 06/18/13 The LeuA146Trp,PheB24Tyr Double Mutant of the Quorum Quenching N-acyl Homoserine Lactone Acylase PvdQ Has an Altered Substrate Specificity Towards Small Acyl Chains PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 99.8
4WKU HYDROLASE/HYDROLASE Inhibitor 10/03/14 n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
3SRA HYDROLASE 07/07/11 Structure of Pseudomonas aerugionsa PvdQ covalently acylated with myristic acid from PVDIq PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 100.0
4K2G HYDROLASE/HYDROLASE INHIBITOR 04/09/13 Structure of Pseudomonas aeruginosa PvdQ bound to BRD-A33442372 PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 100.0
2WYB HYDROLASE 11/16/09 The quorum quenching N-acyl homoserine lactone acylase PvdQ with a covalently bound dodecanoic acid PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 100.0
4K2F HYDROLASE/HYDROLASE INHIBITOR 04/09/13 Structure of Pseudomonas aeruginosa PvdQ bound to BRD-A08522488 PSEUDOMONAS AERUGINOSA 1.99 X-RAY DIFFRACTION 100.0
4WKS HYDROLASE/HYDROLASE INHIBITOR 10/03/14 n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ PSEUDOMONAS AERUGINOSA 1.629 X-RAY DIFFRACTION 100.0
5UBK HYDROLASE 12/20/16 Inactive S1A/N269D-cpPvdQ mutant in complex with the pyoverdine precursor PVDIq reveals a specific binding pocket for the D-Tyr of this substrate PSEUDOMONAS AERUGINOSA 2.55 X-RAY DIFFRACTION 99.6
3SRC HYDROLASE/HYDROLASE INHIBITOR 07/07/11 Structure of Pseudomonas aeruginosa PvdQ bound to NS2028 PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
3L91 HYDROLASE 01/04/10 Structure of Pseudomonas aerugionsa PvdQ bound to octanoate PSEUDOMONAS AERUGINOSA 1.66 X-RAY DIFFRACTION 100.0
3L94 HYDROLASE 01/04/10 Structure of PvdQ covalently acylated with myristate PSEUDOMONAS AERUGINOSA 1.95 X-RAY DIFFRACTION 100.0
3SRB HYDROLASE/HYDROLASE INHIBITOR 07/07/11 Structure of Pseudomonas aeruginosa PvdQ bound to SMER28 PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
2WYD HYDROLASE 11/16/09 The quorum quenching N-acyl homoserine lactone acylase PvdQ in complex with dodecanoic acid PSEUDOMONAS AERUGINOSA 1.901 X-RAY DIFFRACTION 100.0
4WKV HYDROLASE/HYDROLASE Inhibitor 10/03/14 n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ PSEUDOMONAS AERUGINOSA 2.1434 X-RAY DIFFRACTION 100.0
5UBL HYDROLASE 12/20/16 A circularly permuted version of PvdQ (cpPvdQ) PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
2WYE HYDROLASE 11/16/09 The quorum quenching N-acyl homoserine lactone acylase PvdQ is an Ntn- Hydrolase with an unusual substrate-binding pocket PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
2WYC HYDROLASE 11/16/09 The quorum quenching N-acyl homoserine lactone acylase PvdQ in complex with 3-oxo-lauric acid PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 100.0
4M1J HYDROLASE 08/02/13 Crystal structure of Pseudomonas aeruginosa PvdQ in complex with a transition state analogue PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
4WKT HYDROLASE/HYDROLASE Inhibitor 10/03/14 n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ PSEUDOMONAS AERUGINOSA 1.782 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Lauric Acid DB03017 DrugBank 5.0.6 Q9I194 Acyl-homoserine lactone acylase PvdQ 100.0 762 0.0e+00

Virulence Evidence

Source 1
Database VFDB
Category Pyoverdine
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 180 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003142 (485 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2385
Search term: pvdQ

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 39843
Genomic Position 2637570
Position in ORF 1054(2289)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 7914
Genomic Position 2637901
Position in ORF 1385(2289)
Reference 14617778

Transposon Insertions in Orthologs

References

Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa.
Lamont IL, Martin LW
Microbiology (Reading, Engl.) 2003 Apr;149(Pt 4):833-42
PubMed ID: 12686626
Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1.
Sio CF, Otten LG, Cool RH, Diggle SP, Braun PG, Bos R, Daykin M, Cámara M, Williams P, Quax WJ
Infect. Immun. 2006 Mar;74(3):1673-82
PubMed ID: 16495538
The quorum-quenching N-acyl homoserine lactone acylase PvdQ is an Ntn-hydrolase with an unusual substrate-binding pocket.
Bokhove M, Nadal Jimenez P, Quax WJ, Dijkstra BW
Proc. Natl. Acad. Sci. U.S.A. 2010 Jan;107(2):686-91
PubMed ID: 20080736