Pseudomonas aeruginosa PAO1, PA3364 (amiC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3364
Name
amiC
Replicon chromosome
Genomic location 3774578 - 3775735 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_252054.1
GI 15598560
Affymetrix PA3364_amiC_at
Entrez 877798
GenBank AAG06752.1
INSDC AAG06752.1
NCBI Locus Tag PA3364
protein_id(GenBank) gb|AAG06752.1|AE004758_9|gnl|PseudoCAP|PA3364
TIGR NTL03PA03364
UniParc UPI0000165ECF
UniProtKB Acc P27017
UniProtKB ID AMIC_PSEAE
UniRef100 UniRef100_P27017
UniRef50 UniRef50_P27017
UniRef90 UniRef90_P27017

Product

Feature Type CDS
Coding Frame 1
Product Name
aliphatic amidase expression-regulating protein
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.74
Kyte-Doolittle Hydrophobicity Value -0.313
Molecular Weight (kDa) 42.8
Isoelectric Point (pI) 5.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1QO0 BINDING PROTEIN 10/26/99 Amide receptor of the amidase operon of Pseudomonas aeruginosa (AmiC) complexed with the negative regulator AmiR. PSEUDOMONAS AERUGINOSA 2.25 X-RAY DIFFRACTION 98.7
1PEA BINDING PROTEIN 11/16/95 AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH ACETAMIDE PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 98.7
1QNL TRANSFERASE 10/19/99 AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH BUTYRAMIDE PSEUDOMONAS AERUGINOSA 2.7 X-RAY DIFFRACTION 98.4

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Butyramide DB02121 DrugBank 5.0.6 P27017 Aliphatic amidase expression-regulating protein 100.0 385 1.4e-225
Acetamide DB02736 DrugBank 5.0.6 P27017 Aliphatic amidase expression-regulating protein 100.0 385 1.4e-225

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 242 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002264 (358 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3364
Search term: amiC

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 54041
Genomic Position 3775509
Position in ORF 932(1158)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 2322
Genomic Position 3775542
Position in ORF 965(1158)
Reference 14617778

Transposon Insertions in Orthologs

References

Antitermination of amidase expression in Pseudomonas aeruginosa is controlled by a novel cytoplasmic amide-binding protein.
Wilson SA, Wachira SJ, Drew RE, Jones D, Pearl LH
EMBO J. 1993 Sep;12(9):3637-42
PubMed ID: 8253087
Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product.
Wilson S, Drew R
J. Bacteriol. 1991 Aug;173(16):4914-21
PubMed ID: 1907262
Crystal structure of AmiC: the controller of transcription antitermination in the amidase operon of Pseudomonas aeruginosa.
Pearl L, O'Hara B, Drew R, Wilson S
EMBO J. 1994 Dec;13(24):5810-7
PubMed ID: 7813419