Pseudomonas aeruginosa PAO1, PA4221 (fptA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4221
Name
fptA
Replicon chromosome
Genomic location 4724639 - 4726801 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_252911.1
GI 15599417
Affymetrix PA4221_fptA_at
Entrez 880419
GenBank AAG07609.1
INSDC AAG07609.1
NCBI Locus Tag PA4221
protein_id(GenBank) gb|AAG07609.1|AE004839_2|gnl|PseudoCAP|PA4221
TIGR NTL03PA04222
UniParc UPI000012ABC9
UniProtKB Acc P42512
UniProtKB ID FPTA_PSEAE
UniRef100 UniRef100_P42512
UniRef50 UniRef50_P42512
UniRef90 UniRef90_P42512

Product

Feature Type CDS
Coding Frame 1
Product Name
Fe(III)-pyochelin outer membrane receptor precursor
Product Name Confidence: Class 1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.9, molecular weight:80.0, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.85
Kyte-Doolittle Hydrophobicity Value -0.554
Molecular Weight (kDa) 80.0
Isoelectric Point (pI) 6.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1XKW MEMBRANE PROTEIN 09/30/04 Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Lauryl Dimethylamine-N-Oxide DB04147 DrugBank 5.0.6 P42512 Fe(3+)-pyochelin receptor 100.0 720 0.0e+00

Virulence Evidence

Source 1
Database VFDB : VF0095
Category Pyochelin receptor
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 250 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001486 (1085 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4221
Search term: fptA

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 48243
Genomic Position 4725123
Position in ORF 485(2163)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 39215
Genomic Position 4724997
Position in ORF 359(2163)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 39401
Genomic Position 4724996
Position in ORF 358(2163)
Reference 14617778

Transposon Insertions in Orthologs

References

FptA, the Fe(III)-pyochelin receptor of Pseudomonas aeruginosa: a phenolate siderophore receptor homologous to hydroxamate siderophore receptors.
Ankenbauer RG, Quan HN
J. Bacteriol. 1994 Jan;176(2):307-19
PubMed ID: 8288523
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802