Pseudomonas aeruginosa PAO1, PA4418 (ftsI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4418
Name
ftsI
Synonym: pbpB
Replicon chromosome
Genomic location 4952604 - 4954343 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253108.1
GI 15599614
Affymetrix PA4418_ftsI_at
Entrez 881247
GenBank AAG07806.1
INSDC AAG07806.1
NCBI Locus Tag PA4418
protein_id(GenBank) gb|AAG07806.1|AE004856_17|gnl|PseudoCAP|PA4418
TIGR NTL03PA04419
UniParc UPI00000D425F
UniProtKB Acc G3XD46
UniProtKB ID G3XD46_PSEAE
UniRef100 UniRef100_W0YZE8
UniRef50 UniRef50_H8W655
UniRef90 UniRef90_A0A0A8RLY9

Product

Feature Type CDS
Coding Frame 1
Product Name
penicillin-binding protein 3
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 16.14
Kyte-Doolittle Hydrophobicity Value -0.125
Molecular Weight (kDa) 62.9
Isoelectric Point (pI) 10.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4KQR Biosynthetic Protein/Antibiotic 05/15/13 CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH (5S)-Penicilloic Acid PSEUDOMONAS AERUGINOSA 2.01 X-RAY DIFFRACTION 100.0
3PBS HYDROLASE/ANTIBIOTIC 10/20/10 Crystal structure of PBP3 complexed with aztreonam PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
3PBO HYDROLASE/ANTIBIOTIC 10/20/10 Crystal structure of PBP3 complexed with ceftazidime PSEUDOMONAS AERUGINOSA 1.74 X-RAY DIFFRACTION 100.0
3OC2 PENICILLIN-BINDING PROTEIN 08/09/10 Crystal structure of penicillin-binding protein 3 from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.968 X-RAY DIFFRACTION 100.0
5DF8 TRANSFERASE 08/26/15 CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH CEFOPERAZONE PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
4OOM transferase/transferase inhibitor 02/03/14 Crystal structure of PBP3 in complex with BAL30072 ((2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(1,5-dihydroxy-4-oxo-1,4-dihydropyridin-2-yl)methoxy]imino}-N-{(2S)-1-hydroxy-3-methyl-3-[(sulfooxy)amino]butan-2-yl}ethanamide) PSEUDOMONAS AERUGINOSA PA1R 2.0 X-RAY DIFFRACTION 100.0
3OCL PENICILLIN-BINDING PROTEIN/ANTIBIOTIC 08/10/10 Crystal structure of penicillin-binding protein 3 from Pseudomonas aeruginosa in complex with carbenicillin PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 100.0
4KQQ Biosynthetic Protein/Antibiotic 05/15/13 CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH (5S)-Penicilloic Acid PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 100.0
4OOL transferase/transferase inhibitor 02/03/14 Crystal structure of PBP3 in complex with compound 14 ((2E)-2-({[(2S)-2-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(1,5-dihydroxy-4-oxo-1,4-dihydropyridin-2-yl)methoxy]imino}acetyl]amino}-3-oxopropyl]oxy}imino)pentanedioic acid) PSEUDOMONAS AERUGINOSA PA1R 2.3 X-RAY DIFFRACTION 100.0
3PBN HYDROLASE/ANTIBIOTIC 10/20/10 Crystal Structure of Apo PBP3 from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
4WEJ Penicillin-binding protein/inhibitor 09/10/14 Crystal structure of Pseudomonas aeruginosa PBP3 with a R4 substituted allyl monocarbam PSEUDOMONAS AERUGINOSA PA14 2.045 X-RAY DIFFRACTION 100.0
3OCN PENICILLIN-BINDING PROTEIN/ANTIBIOTIC 08/10/10 Crystal structure of penicillin-binding protein 3 from Pseudomonas aeruginosa in complex with ceftazidime PSEUDOMONAS AERUGINOSA 2.61 X-RAY DIFFRACTION 100.0
5DF9 TRANSFERASE 08/26/15 CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 IN COMPLEX WITH DEACYLATED PRODUCT OF CEFOPERAZONE PSEUDOMONAS AERUGINOSA 2.7 X-RAY DIFFRACTION 100.0
3PBR HYDROLASE/ANTIBIOTIC 10/20/10 Crystal structure of PBP3 complexed with meropenem PSEUDOMONAS AERUGINOSA 1.95 X-RAY DIFFRACTION 100.0
4KQO Biosynthetic Protein/Antibiotic 05/15/13 Crystal structure of penicillin-binding protein 3 from pseudomonas aeruginosa in complex with piperacillin PSEUDOMONAS AERUGINOSA 2.31 X-RAY DIFFRACTION 100.0
3PBQ HYDROLASE/ANTIBIOTIC 10/20/10 Crystal structure of PBP3 complexed with imipenem PSEUDOMONAS AERUGINOSA 1.7 X-RAY DIFFRACTION 100.0
4WEL Penicillin-binding protein/inhibitor 09/10/14 Crystal structure of Pseudomonas aeruginosa PBP3 with SMC-3176 PSEUDOMONAS AERUGINOSA PA14 1.99 X-RAY DIFFRACTION 100.0
4FSF PENICILLIN-BINDING PROTEIN/ANTIBIOTIC 06/27/12 Crystal structure of Pseudomonas aeruginosa PBP3 complexed with compound 14 PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 100.0
5DF7 TRANSFERASE 08/26/15 CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH AZLOCILLIN PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
3PBT HYDROLASE/ANTIBIOTIC 10/20/10 Crystal structure of PBP3 complexed with MC-1 PSEUDOMONAS AERUGINOSA 1.641 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Cloxacillin DB01147 DrugBank 5.0.6 Q51504 Cell division protein 100.0 579 0.0e+00
Ceftolozane DB09050 DrugBank 5.0.6 Q51504 Cell division protein 100.0 579 0.0e+00
Cefepime DB01413 DrugBank 5.0.6 Q51504 Cell division protein 100.0 579 0.0e+00
Doripenem DB06211 DrugBank 5.0.6 Q51504 Cell division protein 100.0 579 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 575 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004008 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4418
Search term: ftsI

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 15678
Genomic Position 4952798
Position in ORF 195(1740)
Reference 14617778

Transposon Insertions in Orthologs

References

On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3.
Nakamura M, Maruyama IN, Soma M, Kato J, Suzuki H, Horota Y
Mol. Gen. Genet. 1983 ;191(1):1-9
PubMed ID: 6350821
Binding of penicillin to thiol-penicillin-binding protein 3 of Escherichia coli: identification of its active site.
Houba-Hérin N, Hara H, Inouye M, Hirota Y
Mol. Gen. Genet. 1985 ;201(3):499-504
PubMed ID: 3911028
Membrane topology of penicillin-binding protein 3 of Escherichia coli.
Bowler LD, Spratt BG
Mol. Microbiol. 1989 Sep;3(9):1277-86
PubMed ID: 2677607
Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3.
Liao X, Hancock RE
Antimicrob. Agents Chemother. 1995 Aug;39(8):1871-4
PubMed ID: 7486937