Pseudomonas aeruginosa PAO1, PA5484 (kinB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5484
Name
kinB
Synonym: kinB
Replicon chromosome
Genomic location 6174694 - 6176481 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254171.1
GI 15600677
Affymetrix PA5484_at
Entrez 880850
GenBank AAG08869.1
INSDC AAG08869.1
NCBI Locus Tag PA5484
protein_id(GenBank) gb|AAG08869.1|AE004961_4|gnl|PseudoCAP|PA5484
TIGR NTL03PA05485
UniParc UPI00000C602F
UniProtKB Acc Q9HT87
UniProtKB ID Q9HT87_PSEAE
UniRef100 UniRef100_Q9HT87
UniRef50 UniRef50_O34206
UniRef90 UniRef90_O34206

Product

Feature Type CDS
Coding Frame 1
Product Name
KinB
Product Name Confidence: Class 1
Synonyms histidine kinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (66.00 kDa, PMID:21751344)
Charge (pH 7) -11.89
Kyte-Doolittle Hydrophobicity Value -0.084
Molecular Weight (kDa) 66.0
Isoelectric Point (pI) 5.43

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3KKB TRANSFERASE 11/05/09 The crystal structure of a two-component sensor domain from Pseudomonas aeruginosa PA01 Pseudomonas aeruginosa PAO1 1.881 X-RAY DIFFRACTION 100.0
3L34 TRANSFERASE 12/16/09 The crystal structure of a two-component sensor domain (2nd form) from Pseudomonas aeruginosa PA01 Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0
4LLC TRANSFERASE 07/09/13 The crystal structure of R60E mutant of the histidine kinase (KinB) sensor domain from Pseudomonas aeruginosa PA01 Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 99.2
4LLE TRANSFERASE 07/09/13 The crystal structure of R60L mutant of the histidine kinase (KinB) sensor domain from Pseudomonas aeruginosa PA01 Pseudomonas aeruginosa 1.68 X-RAY DIFFRACTION 99.2
3N24 TRANSFERASE 05/17/10 The crystal structure of a two-component sensor domain (3rd form) from Pseudomonas aeruginosa PA01 Pseudomonas aeruginosa 2.056 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 622 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005024 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5484
Search term: kinB
Search term: KinB

Human Homologs

References

Identification of the histidine protein kinase KinB in Pseudomonas aeruginosa and its phosphorylation of the alginate regulator algB.
Ma S, Wozniak DJ, Ohman DE
J Biol Chem 1997 Jul 18;272(29):17952-60
PubMed ID: 9218420
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Lipotoxin F of Pseudomonas aeruginosa is an AlgU-dependent and alginate-independent outer membrane protein involved in resistance to oxidative stress and adhesion to A549 human lung epithelia.
Damron FH, Napper J, Teter MA, Yu HD
Microbiology (Reading) 2009 Apr;155(Pt 4):1028-1038
PubMed ID: 19332805
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J Bacteriol 2009 Apr;191(7):2285-95
PubMed ID: 19168621
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344