Pseudomonas aeruginosa PAO1, PA0086 (tagJ1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0086
Name
tagJ1
Synonym: hsiE1
Replicon chromosome
Genomic location 104006 - 104851 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248776.1
GI 15595284
Affymetrix PA0086_at
DNASU PaCD00006038
Entrez 879504
GenBank AAG03476.1
INSDC AAG03476.1
NCBI Locus Tag PA0086
protein_id(GenBank) gb|AAG03476.1|AE004447_9|gnl|PseudoCAP|PA0086
TIGR NTL03PA00087
UniParc UPI00000C4F27
UniProtKB Acc Q9I746
UniProtKB ID Q9I746_PSEAE
UniRef100 UniRef100_Q9I746
UniRef50 UniRef50_A1KCF9
UniRef90 UniRef90_Q9I746

Product

Feature Type CDS
Coding Frame 1
Product Name
TagJ1
Product Name Confidence: Class 1
CommentHcp Secretion Island-I-encoded type VI secretion system (H1-T6SS)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.54
Kyte-Doolittle Hydrophobicity Value 0.019
Molecular Weight (kDa) 30.4
Isoelectric Point (pI) 4.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4UQY PROTEIN TRANSPORT 06/25/14 Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes PSEUDOMONAS AERUGINOSA PAO1; SYNTHETIC CONSTRUCT 1.6 X-RAY DIFFRACTION 100.0
4UQX PROTEIN TRANSPORT 06/25/14 Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes PSEUDOMONAS AERUGINOSA PAO1 1.2 X-RAY DIFFRACTION 100.0
4UQZ PROTEIN TRANSPORT 06/25/14 Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes PSEUDOMONAS AERUGINOSA PAO1 1.599 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 44 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000085 (310 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0086
Search term: tagJ1
Search term: TagJ1

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433
PubMed ID: 28673999
PubMed ID: 25305017
PubMed ID: 22906320