Pseudomonas aeruginosa PAO1, PA0262 (vgrG2b)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0262
Name
vgrG2b
Replicon chromosome
Genomic location 293802 - 296861 (- strand)
Transposon Mutants 13 transposon mutants in PAO1
Transposon Mutants in orthologs 10 transposon mutants in orthologs
CommentTli3 neutralizes the antibacterial activity of VgrG2b (PMID:31231326).
Comment The C-Terminal domain of VgrG2b interacts with the gamma-tubulin complex to promote a microtubule-dependant internalization of P. aeruginosa into non phagocytic cells (PMID:26037124)
CommentH2-T6SS evolved effector demonstrating antibacterial and anti-eukaryotic activity (trans-kingdom toxin) delivered into epithelial cells through the H2-T6SS machinery and VgrG2a (PA1511)
Comment Required for Tle3 secretion, the C-Terminal domain of VgrG2b is antibacterial when delivered into the periplasm of prey bacteria (in the heterologous host E. coli) and its toxicity relies on an intact protease catalytic site (residus 932-941, PFAM04298)

Cross-References

RefSeq NP_248953.1
GI 15595459
Affymetrix PA0262_at
Entrez 881981
GenBank AAG03651.1
INSDC AAG03651.1
NCBI Locus Tag PA0262
protein_id(GenBank) gb|AAG03651.1|AE004464_4|gnl|PseudoCAP|PA0262
TIGR NTL03PA00263
UniParc UPI00000C4FCB
UniProtKB Acc Q9I6M7
UniProtKB ID Q9I6M7_PSEAE
UniRef100 UniRef100_Q9I6M7
UniRef50 UniRef50_Q9I3K1
UniRef90 UniRef90_Q9I3K1

Product

Feature Type CDS
Coding Frame 1
Product Name
VgrG2b
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -19.34
Kyte-Doolittle Hydrophobicity Value -0.478
Molecular Weight (kDa) 113.0
Isoelectric Point (pI) 6.16

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Host Associated Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6H56 METAL BINDING PROTEIN 07/23/18 Effector domain of Pseudomonas aeruginosa VgrG2b PSEUDOMONAS AERUGINOSA (ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.2 X-RAY DIFFRACTION 98.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 146 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000090 (3404 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0262
Search term: vgrG2b
Search term: VgrG2b

References

Rhs elements comprise three subfamilies which diverged prior to acquisition by Escherichia coli.
Wang YD, Zhao S, Hill CW
J. Bacteriol. 1998 Aug;180(16):4102-10
PubMed ID: 9696756
Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins.
Hachani A, Lossi NS, Hamilton A, Jones C, Bleves S, Albesa-Jové D, Filloux A
J. Biol. Chem. 2011 Apr;286(14):12317-27
PubMed ID: 21325275
Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network.
Sana TG, Baumann C, Merdes A, Soscia C, Rattei T, Hachani A, Jones C, Bennett KL, Filloux A, Superti-Furga G, Voulhoux R, Bleves S.
MBio. 2015 Jun 2;6(3):e00712.
PubMed ID: 26037124
A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in <i>Pseudomonas aeruginosa</i>.
Berni B, Soscia C, Djermoun S, Ize B, Bleves S
Front Microbiol 2019;10:1218
PubMed ID: 31231326