Pseudomonas aeruginosa PAO1, PA1148 (toxA)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA1148
|
| Name |
toxA
Synonym: eta |
| Replicon | chromosome |
| Genomic location | 1240584 - 1242500 (- strand) |
| Transposon Mutants | 2 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_249839.1 |
| GI | 15596345 |
| Affymetrix | PA1148_toxA_at |
| Entrez | 877850 |
| GenBank | AAG04537.1 |
| INSDC | AAG04537.1 |
| NCBI Locus Tag | PA1148 |
| protein_id(GenBank) | gb|AAG04537.1|AE004544_9|gnl|PseudoCAP|PA1148 |
| TIGR | NTL03PA01149 |
| UniParc | UPI0000137188 |
| UniProtKB Acc | P11439 |
| UniProtKB ID | TOXA_PSEAE |
| UniRef100 | UniRef100_P11439 |
| UniRef50 | UniRef50_P11439 |
| UniRef90 | UniRef90_P11439 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
exotoxin A precursor
|
| Synonyms | |
| Evidence for Translation | |
| Charge (pH 7) | -16.12 |
| Kyte-Doolittle Hydrophobicity Value | -0.262 |
| Molecular Weight (kDa) | 69.3 |
| Isoelectric Point (pI) | 5.29 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 1DMA | ADP-RIBOSYLATION | 04/28/95 | DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH NICOTINAMIDE AND AMP | Pseudomonas aeruginosa | 2.5 | X-RAY DIFFRACTION | 99.1 |
| 6EDG | TRANSFERASE | 08/09/18 | Pseudomonas exotoxin A domain III T18H477L | Pseudomonas aeruginosa | 1.47 | X-RAY DIFFRACTION | 96.7 |
| 1IKP | TRANSFERASE | 05/04/01 | Pseudomonas Aeruginosa Exotoxin A, P201Q, W281A mutant | Pseudomonas aeruginosa | 1.45 | X-RAY DIFFRACTION | 99.2 |
| 1ZM4 | BIOSYNTHETIC PROTEIN/TRANSFERASE | 05/10/05 | Structure of the eEF2-ETA-bTAD complex | Pseudomonas aeruginosa; Saccharomyces cerevisiae | 2.9 | X-RAY DIFFRACTION | 99.0 |
| 1AER | ADP-RIBOSYLATION | 12/11/95 | DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH BETA-TAD | Pseudomonas aeruginosa | 2.3 | X-RAY DIFFRACTION | 99.0 |
| 1ZM2 | BIOSYNTHETIC PROTEIN/TRANSFERASE | 05/10/05 | Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA | Pseudomonas aeruginosa; Saccharomyces cerevisiae | 3.07 | X-RAY DIFFRACTION | 99.0 |
| 1ZM9 | BIOSYNTHETIC PROTEIN/TRANSFERASE | 05/10/05 | Structure of eEF2-ETA in complex with PJ34 | Pseudomonas aeruginosa; Saccharomyces cerevisiae | 2.8 | X-RAY DIFFRACTION | 99.0 |
| 2ZIT | BIOSYNTHETIC PROTEIN/TRANSFERASE | 02/24/08 | Structure of the eEF2-ExoA-NAD+ complex | Pseudomonas aeruginosa; Saccharomyces cerevisiae | 3 | X-RAY DIFFRACTION | 99.0 |
| 1IKQ | TRANSFERASE | 05/04/01 | Pseudomonas Aeruginosa Exotoxin A, wild type | Pseudomonas aeruginosa | 1.62 | X-RAY DIFFRACTION | 99.5 |
| 1ZM3 | BIOSYNTHETIC PROTEIN/TRANSFERASE | 05/10/05 | Structure of the apo eEF2-ETA complex | Pseudomonas aeruginosa; Saccharomyces cerevisiae | 3.07 | X-RAY DIFFRACTION | 99.0 |
| 1XK9 | TRANSFERASE | 09/28/04 | Pseudomanas exotoxin A in complex with the PJ34 inhibitor | Pseudomonas aeruginosa | 2.1 | X-RAY DIFFRACTION | 99.1 |
Drugs Targeting this Protein
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
| Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
| N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE | DB08348 | DrugBank | 5.1.4 | P11439 | Exotoxin A | 100.0 | 638 | 0.0e+00 |
| Nicotinamide | DB02701 | DrugBank | 5.1.4 | P11439 | Exotoxin A | 100.0 | 638 | 0.0e+00 |
Virulence Evidence
| Source 1 | |
| Database | VFDB : VF0086 |
| Category | Exototoxin A |
| Evidence |
|
| Host Organism | |
| Notes | |
| Reference | 12270928 |
| Source 2 | |
| Database | Victors : 4206 |
| Category | Exototoxin A |
| Evidence |
ECO:0000314
direct assay evidence used in manual assertion
|
| Host Organism | Mus musculus |
| Notes | toxA (exotoxin A) blocks protein synthesis by ADP ribosylation of elongation factor 2, thereby triggering cell death |
| Reference | 19380469 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG001108 (295 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA1148
Search term: toxA
Search term: exotoxin A precursor
|
Human Homologs
References
|
Mechanism of protein excretion by gram-negative bacteria: Pseudomonas aeruginosa exotoxin A.
Lory S, Tai PC, Davis BD
J Bacteriol 1983 Nov;156(2):695-702
PubMed ID: 6415037
|
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
|
Cloning, nucleotide sequence, and expression in Escherichia coli of the exotoxin A structural gene of Pseudomonas aeruginosa.
Gray GL, Smith DH, Baldridge JS, Harkins RN, Vasil ML, Chen EY, Heyneker HL
Proc Natl Acad Sci U S A 1984 May;81(9):2645-9
PubMed ID: 6201861
|