Pseudomonas aeruginosa PAO1, PA1150 (pys2)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 1243594 - 1245663 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_249841.1
GI 15596347
Affymetrix PA1150_pys2_at
Entrez 878080
GenBank AAG04539.1
INSDC AAG04539.1
NCBI Locus Tag PA1150
protein_id(GenBank) gb|AAG04539.1|AE004545_2|gnl|PseudoCAP|PA1150
UniParc UPI0000165EC2
UniProtKB Acc Q06584
UniRef100 UniRef100_Q06584
UniRef50 UniRef50_Q06584
UniRef90 UniRef90_Q06584


Feature Type CDS
Coding Frame 1
Product Name
pyocin S2
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (73.85 kDa, PMID:21751344)
Charge (pH 7) 9.71
Kyte-Doolittle Hydrophobicity Value -0.360
Molecular Weight (kDa) 73.9
Isoelectric Point (pI) 9.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Extracellular Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4QKO ANTIMICROBIAL PROTEIN 06/07/14 The Crystal Structure of the Pyocin S2 Nuclease Domain, Immunity Protein Complex at 1.8 Angstroms PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
5ODW PROTEIN TRANSPORT 07/06/17 Structure of the FpvAI-pyocin S2 complex PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PR 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000948 (432 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1150
Search term: pys2
Search term: pyocin S2


Purification of the pyocin S2 complex from Pseudomonas aeruginosa PAO1: analysis of DNase activity.
Seo Y, Galloway DR
Biochem. Biophys. Res. Commun. 1990 Oct;172(2):455-61
PubMed ID: 2122894
Molecular structures and functions of pyocins S1 and S2 in Pseudomonas aeruginosa.
Sano Y, Matsui H, Kobayashi M, Kageyama M
J. Bacteriol. 1993 May;175(10):2907-16
PubMed ID: 8491711
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344