Pseudomonas aeruginosa PAO1, PA1510 (tplE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1510
Name
tplE
Synonym: tle4
Replicon chromosome
Genomic location 1639791 - 1641500 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250201.1
GI 15596707
Affymetrix PA1510_at
Entrez 879265
GenBank AAG04899.1
INSDC AAG04899.1
NCBI Locus Tag PA1510
protein_id(GenBank) gb|AAG04899.1|AE004579_11|gnl|PseudoCAP|PA1510
TIGR NTL03PA01511
UniParc UPI00000C53B7
UniProtKB Acc Q9I3K2
UniProtKB ID Q9I3K2_PSEAE
UniRef100 UniRef100_Q9I3K2
UniRef50 UniRef50_Q9I3K2
UniRef90 UniRef90_Q9I3K2

Product

Feature Type CDS
Coding Frame 1
Product Name
type 6 PGAP1-like effector, TplE
Product Name Confidence: Class 2
Synonyms Tle4
Evidence for Translation
Charge (pH 7) -14.21
Kyte-Doolittle Hydrophobicity Value -0.636
Molecular Weight (kDa) 63.3
Isoelectric Point (pI) 5.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R1D HYDROLASE/HYDROLASE INHIBITOR 08/05/14 The crystal structure of Tle4-Tli4 complex Pseudomonas aeruginosa PAO1 1.749 X-RAY DIFFRACTION 100.0
5H7Y HYDROLASE INHIBITOR/PEPTIDE 11/21/16 Structure of immunity protein TplEi of T6SS from Pseudomonas aeruginosa complexed with L peptide Pseudomonas aeruginosa PAO1 2.195 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003845 (309 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1510
Search term: tplE

Human Homologs

References

Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
Nature 2013 Apr 25;496(7446):508-12
PubMed ID: 23552891
The Pseudomonas aeruginosa Type VI Secretion PGAP1-like Effector Induces Host Autophagy by Activating Endoplasmic Reticulum Stress.
Jiang F, Wang X, Wang B, Chen L, Zhao Z, Waterfield NR, Yang G, Jin Q
Cell Rep 2016 Aug 9;16(6):1502-1509
PubMed ID: 27477276