Pseudomonas aeruginosa PAO1, PA1659 (hsiF2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1659
Name
hsiF2
Synonym: tssE2
Replicon chromosome
Genomic location 1807241 - 1807648 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250350.1
GI 15596856
Affymetrix PA1659_at
Entrez 880734
GenBank AAG05048.1
INSDC AAG05048.1
NCBI Locus Tag PA1659
protein_id(GenBank) gb|AAG05048.1|AE004593_10|gnl|PseudoCAP|PA1659
TIGR NTL03PA01660
UniParc UPI00000C5431
UniProtKB Acc Q9I366
UniProtKB ID Q9I366_PSEAE
UniRef100 UniRef100_Q9I366
UniRef50 UniRef50_Q9I366
UniRef90 UniRef90_Q9I366

Product

Feature Type CDS
Coding Frame 1
Product Name
HsiF2
Product Name Confidence: Class 1
CommentHcp Secretion Island-II-encoded type VI secretion system (H2-T6SS)
Evidence for Translation
Charge (pH 7) -2.56
Kyte-Doolittle Hydrophobicity Value -0.187
Molecular Weight (kDa) 14.7
Isoelectric Point (pI) 5.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000317
genomic context evidence used in manual assertion
Host Organism Homo sapiens
Notes H2-T6SS rendered inactive via clpV2 deletion.
Reference 22665491

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 48 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000086 (851 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1659
Search term: hsiF2
Search term: HsiF2

References

Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa.
Lossi NS, Dajani R, Freemont P, Filloux A.
Microbiology. 2011 Dec;157(Pt 12):3292-305.
PubMed ID: 21873404
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999