Pseudomonas aeruginosa PAO1, PA2012 (liuD)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: mccA
Synonym: gnyA
Synonym: accA
Replicon chromosome
Genomic location 2200685 - 2202652 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_250702.1
GI 15597208
Affymetrix PA2012_at
Entrez 879012
GenBank AAG05400.1
INSDC AAG05400.1
NCBI Locus Tag PA2012
protein_id(GenBank) gb|AAG05400.1|AE004627_8|gnl|PseudoCAP|PA2012
UniParc UPI00000C5558
UniProtKB Acc Q9I299
UniRef100 UniRef100_Q9I299
UniRef50 UniRef50_Q9I299
UniRef90 UniRef90_Q9I299


Feature Type CDS
Coding Frame 1
Product Name
methylcrotonyl-CoA carboxylase, alpha-subunit (biotin-containing)
Product Name Confidence: Class 1
Synonyms MccA
alpha subunit of geranoyl-CoA carboxylase, GnyA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.10
Kyte-Doolittle Hydrophobicity Value -0.234
Molecular Weight (kDa) 71.3
Isoelectric Point (pI) 5.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3U9S LIGASE 10/19/11 Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC) 750 kD holoenzyme, CoA complex PSEUDOMONAS AERUGINOSA 3.5 X-RAY DIFFRACTION 100.0
3U9T LIGASE 10/19/11 Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC) 750 kD holoenzyme, free enzyme PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 643 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003427 (539 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2012
Search term: liuD

Human Homologs

Ensembl 94, assembly GRCh38.p12
methylcrotonoyl-CoA carboxylase 1 [Source:HGNC Symbol;Acc:HGNC:6936]
E-value: 3.5e-163
Percent Identity: 45.9


The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa.
Díaz-Pérez AL, Zavala-Hernández AN, Cervantes C, Campos-García J
Appl. Environ. Microbiol. 2004 Sep;70(9):5102-10
PubMed ID: 15345388
Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa.
Förster-Fromme K, Höschle B, Mack C, Bott M, Armbruster W, Jendrossek D
Appl. Environ. Microbiol. 2006 Jul;72(7):4819-28
PubMed ID: 16820476
Lipid synthesis in mycobacteria: characterization of the biotin carboxyl carrier protein genes from Mycobacterium leprae and M. tuberculosis.
Norman E, De Smet KA, Stoker NG, Ratledge C, Wheeler PR, Dale JW
J. Bacteriol. 1994 May;176(9):2525-31
PubMed ID: 7909542