Pseudomonas aeruginosa PAO1, PA2018 (mexY)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: mexH
Synonym: amrB
Replicon chromosome
Genomic location 2208169 - 2211306 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 8 transposon mutants in orthologs


RefSeq NP_250708.1
GI 15597214
Affymetrix PA2018_at
Entrez 878882
GenBank AAG05406.1
INSDC AAG05406.1
NCBI Locus Tag PA2018
protein_id(GenBank) gb|AAG05406.1|AE004628_4|gnl|PseudoCAP|PA2018
UniParc UPI00000D420B
UniProtKB Acc G3XCW2
UniRef100 UniRef100_G3XCW2
UniRef50 UniRef50_P31224
UniRef90 UniRef90_G3XCW2


Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexY
Product Name Confidence: Class 1
Synonyms RND multidrug efflux transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.58
Kyte-Doolittle Hydrophobicity Value 0.341
Molecular Weight (kDa) 112.7
Isoelectric Point (pI) 6.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
amrB amrB is the membrane fusion protein of the AmrAB-OprM multidrug efflux complex. ARO:3002983 protein homolog model Perfect 0.0 1.0 1.1.8

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000417 (2173 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2018
Search term: mexY


Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei.
Moore RA, DeShazer D, Reckseidler S, Weissman A, Woods DE
Antimicrob. Agents Chemother. 1999 Mar;43(3):465-70
PubMed ID: 10049252
MexXY multidrug efflux system of Pseudomonas aeruginosa.
Morita Y, Tomida J, Kawamura Y
Front Microbiol 2012 ;3
PubMed ID: 23233851
Involvement of an active efflux system in the natural resistance of Pseudomonas aeruginosa to aminoglycosides.
Aires JR, Köhler T, Nikaido H, Plésiat P
Antimicrob. Agents Chemother. 1999 Nov;43(11):2624-8
PubMed ID: 10543738
Contribution of the MexX-MexY-oprM efflux system to intrinsic resistance in Pseudomonas aeruginosa.
Masuda N, Sakagawa E, Ohya S, Gotoh N, Tsujimoto H, Nishino T
Antimicrob. Agents Chemother. 2000 Sep;44(9):2242-6
PubMed ID: 10952562
Induction of the MexXY efflux pump in Pseudomonas aeruginosa is dependent on drug-ribosome interaction.
Jeannot K, Sobel ML, El Garch F, Poole K, Plésiat P
J. Bacteriol. 2005 Aug;187(15):5341-6
PubMed ID: 16030228